Purinergic receptor antagonists inhibit odorant-mediated CREB phosphorylation in sustentacular cells of mouse olfactory epithelium

BMC Neuroscience

Research Article

Hanns Hatt¹  Ruth Dooley²  Anastasia Mashukova³  Eva M Neuhaus⁴  Bastian Toetter⁴ 

[1] Department of Cell Physiology, Ruhr-University Bochum, Universitätsstrasse 150, 44780, Bochum, Germany;Department of Molecular Medicine, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin, Ireland;Department of Physiology, College of Medical Sciences, Nova Southeastern University, 33328, FL, USA;NeuroScience Research Center, Charité - Universitätsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Germany;

关键词: Olfactory Epithelium;    cAMP Response Element Binding;    Purinergic Receptor;    Olfactory Sensory Neuron;    Sustentacular Cell;   

DOI  :  10.1186/1471-2202-12-86

 received in 2011-02-17, accepted in 2011-08-22,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundExtracellular nucleotides have long been known to play neuromodulatory roles and to be involved in intercellular signalling. In the olfactory system, ATP is released by olfactory neurons, and exogenous ATP can evoke an increase in intracellular calcium concentration in sustentacular cells, the nonneuronal supporting cells of the olfactory epithelium. Here we investigate the hypothesis that olfactory neurons communicate with sustentacular cells via extracellular ATP and purinergic receptor activation.ResultsHere we show that exposure of mice to a mixture of odorants induced a significant increase in the levels of the transcription factor CREB phosphorylated at Ser-133 in the nuclei of both olfactory sensory neurons and sustentacular cells. This activation was dependent on adenylyl cyclase III-mediated olfactory signaling and on activation of P2Y purinergic receptors on sustentacular cells. Purinergic receptor antagonists inhibited odorant-dependent CREB phosphorylation specifically in the nuclei of the sustentacular cells.ConclusionOur results point to a possible role for extracellular nucleotides in mediating intercellular communication between the neurons and sustentacular cells of the olfactory epithelium in response to odorant exposure. Maintenance of extracellular ionic gradients and metabolism of noxious chemicals by sustentacular cells may therefore be regulated in an odorant-dependent manner by olfactory sensory neurons.

【 授权许可】

Unknown   
© Dooley et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.