A comparative analysis of the response of the hepatic transcriptome to dietary docosahexaenoic acid in Atlantic salmon (Salmo salar) post-smolts

BMC Genomics

Research Article

Brett D. Glencross¹  Monica B. Betancor²  Douglas R. Tocher²  Christian De Santis²  John B. Taggart²  Beatrix Bicskei²  James E. Bron² 

[1] CSIRO Aquaculture, GPO Box 2583, 4001, Brisbane, QLD, Australia;Institute of Aquaculture, School of Natural Sciences, Stirling University, FK9 4LA, Stirling, Scotland, UK;

关键词: Essential fatty acid;    DHA;    Gene expression;    Transcriptome;    Atlantic salmon;    Microarray;   

DOI  :  10.1186/s12864-015-1810-z

 received in 2015-01-20, accepted in 2015-08-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe present study aimed to explore the impact of dietary docosahexaenoic acid (DHA) on aspects of the metabolism of Atlantic salmon (Salmo salar). The effects of diets containing increasing levels of DHA (1 g kg−1, 3 g kg−1, 6 g kg−1, 10 g kg−1 and 13 g kg−1) on the liver transcriptome of post-smolt salmon was examined to elucidate patterns of gene expression and responses of specific metabolic pathways. Total RNA was isolated from the liver of individual fish and analyzed using a custom gene expression 44K feature Atlantic salmon oligo-microarray.ResultsThe expression of up to 911 unique annotated genes was significantly affected by dietary DHA inclusion relative to a low DHA reference diet. Analysis of a total of 797 unique genes were found with a significant linear correlation between expression level and dietary DHA. Gene-Set Enrichment Analysis (GSEA) identified a range of pathways that were significantly affected by dietary DHA content.ConclusionsPathways that showed a significant response to dietary DHA level included those for long-chain polyunsaturated fatty acid biosynthesis, fatty acid elongation, steroid biosynthesis, glycan biosynthesis, protein export and protein processing in the endoplasmic reticulum. These findings suggest that in addition to clear roles in influencing lipid metabolic pathways, DHA might also have key functional roles in other pathways distinct from lipid metabolism.

【 授权许可】

CC BY   
© Glencross et al. 2015