Genome-wide association study identifies a major gene for beech bark disease resistance in American beech (Fagus grandifolia Ehrh.)

BMC Genomics

Research Article

Charles Addo-Quaye¹  Irina Ćalić²  David B. Neale²  John E. Carlson³  David Carey⁴  Jennifer Koch⁴ 

[1] Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, 47907, Indiana, USA;Present address: Division of Natural Sciences and Mathematics, Lewis-Clark State College, 500 8th Avenue, 83501, Lewiston, ID, USA;Department of Plant Sciences, University of California, 95616, Davis, CA, USA;Schatz Center for Tree Molecular Genetics, Department of Ecosystem Science and Management, Pennsylvania State University, 16802, University Park, PA, USA;USDA Forest Service, Northern Research Station, Forestry Sciences Laboratory, 43015, Delaware, OH, USA;

关键词: Beech bark disease;    Association mapping;    Resistance genes;    American beech;   

DOI  :  10.1186/s12864-017-3931-z

 received in 2017-01-09, accepted in 2017-07-07,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe American Beech tree (Fagus grandifolia Ehrh.), native to eastern North America, is ecologically important and provides high quality wood products. This species is susceptible to beech bark disease (BBD) and is facing high rates of mortality in North America. The disease occurs from an interaction between the woolly beech scale insect (Cryptococcus fagisuga), one of two species of the fungus Neonectria (N. faginata or N. ditissima), and American Beech trees.MethodsIn this case-control genome-wide association study (GWAS), we tested 16 K high quality SNPs using the Affymetrix Axiom 1.5 K – 50 K assay to genotype an association population of 514 individuals. We also conducted linkage analysis in a full-sib family of 115 individuals. Fisher’s exact test and logistic regression tests were performed to test associations between SNPs and phenotypes.ResultsAssociation tests revealed four highly significant SNPs on chromosome (Chr) 5 for a single gene (Mt), which encodes a mRNA for metallothionein-like protein (metal ion binding) in Fagus sylvatica. Metallothioneins represent Cys-rich metal chelators able to coordinate metal atoms and may play an important role in the resistance mechanisms against beech scale insect.ConclusionThe GWAS study has identified a single locus of major effect contributing to beech bark disease resistance. Knowledge of this genetic locus contributing to resistance might be used in applied breeding, conservation and restoration programs.

【 授权许可】

CC BY   
© The Author(s). 2017