Evaluation of the malaria rapid diagnostic test VIKIA malaria Ag Pf/Pan™ in endemic and non-endemic settings

Malaria Journal

Research

Boubacar Coulibaly¹  Boubacar Traore¹  Nianégué Coulibaly¹  Fanta Siby²  Daniel Eibach³  Anne-Lise Bienvenu⁴  Stéphane Picot⁴  Mourad Bouchrik⁵  Guillaume Bonnot⁶ 

[1] Centre de Santé de Référence, Commune VI, Bamako, Mali;Direction Régionale de la Santé, Ministry of Health, Bamako, Mali;Institut de Parasitologie et de Mycologie Médicale, Faculty of Medicine, Hospices Civils de Lyon, Lyon, France;Institut de Parasitologie et de Mycologie Médicale, Faculty of Medicine, Hospices Civils de Lyon, Lyon, France;Malaria Research Unit, CNRS UMR 5246, University Lyon 1, Lyon, France;Laboratoire de Parasitology & Mycology, Faculty of Medicine, Hôpital Militaire Mohamed V, Rabat, Morocco;Malaria Research Unit, CNRS UMR 5246, University Lyon 1, Lyon, France;

关键词: Malaria;    Rapid diagnostic test;    Diagnosis;    Mali;    Sensitivity/specificity;   

DOI  :  10.1186/1475-2875-12-188

 received in 2013-01-21, accepted in 2013-06-03,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundMalaria rapid diagnostic tests (RDTs) are a useful tool in endemic malaria countries, where light microscopy is not feasible. In non-endemic countries they can be used as complementary tests to provide timely results in case of microscopy inexperience. This study aims to compare the new VIKIA Malaria Ag Pf/Pan™ RDT with PCR-corrected microscopy results and the commonly used CareStart™ RDT to diagnose falciparum and non-falciparum malaria in the endemic setting of Bamako, Mali and the non-endemic setting of Lyon, France.MethodsBlood samples were collected during a 12-months and six-months period in 2011 from patients suspected to have malaria in Lyon and Bamako respectively. The samples were examined by light microscopy, the VIKIA Malaria Ag Pf/Pan™ test and in Bamako additionally with the CareStart™ RDT. Discordant results were corrected by real-time PCR. Sensitivity, specificity, positive predictive value and negative predictive value were used to evaluate test performance.ResultsSamples of 877 patients from both sites were included. The VIKIA Malaria Ag Pf/Pan™ had a sensitivity of 98% and 96% for Plasmodium falciparum in Lyon and Bamako, respectively, performing similar to PCR-corrected microscopy.ConclusionsThe VIKIA Malaria Ag Pf/Pan™ performs similar to PCR-corrected microscopy for the detection of P. falciparum, making it a valuable tool in malaria endemic and non-endemic regions.

【 授权许可】

CC BY   
© Eibach et al.; licensee BioMed Central Ltd. 2013