Diversity of malaria parasites in great apes in Gabon

Malaria Journal

Research

Alain Prince Okouga¹  Benjamin Ollomo¹  Nancy Diamella Moukodoum¹  Lucrèce M Delicat-Loembet¹  Guillaume Le Flohic¹  Alphonse Ndoungouet¹  Lauriane Yacka Mouele¹  Vanina Boué¹  Bertrand Mve-Ondo¹  Peggy Motsch¹  Larson Boundenga²  Virginie Rougeron³  Christophe Paupy³  Franck Prugnolle³  Florian Liégeois⁴  Cheikh Tidiane Ba⁵  Patrick Durand⁶  Eric Elguero⁶  Francois Renaud⁶  Céline Arnathau⁶ 

[1] Centre International de Recherche Médicale de Franceville, BP 769, Franceville-Gabon, Gabon;Centre International de Recherche Médicale de Franceville, BP 769, Franceville-Gabon, Gabon;Laboratoire d’Écologie et Biologie évolutive, Département de Biologie Animale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar, BP5005, Dakar, Sénégal;Centre International de Recherche Médicale de Franceville, BP 769, Franceville-Gabon, Gabon;MIVEGEC (Laboratoire Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle), UMR CNRS 5290 / IRD 224, Université Montpellier 1, Montpellier, France;Centre International de Recherche Médicale de Franceville, BP 769, Franceville-Gabon, Gabon;TransVIHMI (Recherche Translationnelle sur le VIH et les Maladies Infectieuses), UMI 233, Institut de Recherche pour le Développement (IRD) and Université Montpellier 1, Montpellier, France;Laboratoire d’Écologie et Biologie évolutive, Département de Biologie Animale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar, BP5005, Dakar, Sénégal;MIVEGEC (Laboratoire Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle), UMR CNRS 5290 / IRD 224, Université Montpellier 1, Montpellier, France;

关键词: Plasmodial diversity;    Laverania;    Great apes;    Cytochrome-b;    Gabon;   

DOI  :  10.1186/s12936-015-0622-6

 received in 2014-11-20, accepted in 2015-02-22,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundUntil 2009, the Laverania subgenus counted only two representatives: Plasmodium falciparum and Plasmodium reichenowi. The recent development of non-invasive methods allowed re-exploration of plasmodial diversity in African apes. Although a large number of great ape populations have now been studied regarding Plasmodium infections in Africa, there are still vast areas of their distribution that remained unexplored. Gabon constitutes an important part of the range of western central African great ape subspecies (Pan troglodytes troglodytes and Gorilla gorilla gorilla), but has not been studied so far. In the present study, the diversity of Plasmodium species circulating in great apes in Gabon was analysed.MethodsThe analysis of 1,261 faecal samples from 791 chimpanzees and 470 gorillas collected from 24 sites all over Gabon was performed. Plasmodium infections were characterized by amplification and sequencing of a portion of the Plasmodium cytochrome b gene.ResultsThe analysis of the 1,261 samples revealed that at least six Plasmodium species circulate in great apes in Gabon (Plasmodium praefalciparum, Plasmodium gorA (syn Plasmodium adleri), Plasmodium gorB (syn Plasmodium blacklocki) in gorillas and Plasmodium gaboni, P. reichenowi and Plasmodium billcollinsi in chimpanzees). No new phylogenetic lineages were discovered. The average infection rate was 21.3% for gorillas and 15.4% for chimpanzees. A logistic regression showed that the probability of infection was significantly dependent on the freshness of the droppings but not of the host species or of the average pluviometry of the months of collection.

【 授权许可】

CC BY   
© Boundenga et al.; licensee BioMed Central. 2015