Genome-level identification, gene expression, and comparative analysis of porcine β-defensin genes

BMC Genetics

Research Article

Jiyeon Hyeon¹  Jungwan Chun¹  Kunho Seo¹  Won Kim²  Dinh Truong Nguyen²  Minh Thong Le²  Chankyu Park²  Min-Kyeung Choi²  Hojun Choi²  Jin-Hoi Kim² 

[1]College of Veterinary Medicine, Konkuk University, Seoul, South Korea

[2]Department of Animal Biotechnology, Konkuk University, Hwayang-dong, 143-701, Kwangjin-gu, Seoul, South Korea

关键词: Antimicrobial peptide;    β-defensins;    Pigs;    pBD;    Pig genome;    SNP;   

DOI  :  10.1186/1471-2156-13-98

 received in 2012-03-06, accepted in 2012-07-19,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundBeta-defensins (β-defensins) are innate immune peptides with evolutionary conservation across a wide range of species and has been suggested to play important roles in innate immune reactions against pathogens. However, the complete β-defensin repertoire in the pig has not been fully addressed.ResultA BLAST analysis was performed against the available pig genomic sequence in the NCBI database to identify β-defensin-related sequences using previously reported β-defensin sequences of pigs, humans, and cattle. The porcine β-defensin gene clusters were mapped to chromosomes 7, 14, 15 and 17. The gene expression analysis of 17 newly annotated porcine β-defensin genes across 15 tissues using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) showed differences in their tissue distribution, with the kidney and testis having the largest pBD expression repertoire. We also analyzed single nucleotide polymorphisms (SNPs) in the mature peptide region of pBD genes from 35 pigs of 7 breeds. We found 8 cSNPs in 7 pBDs.ConclusionWe identified 29 porcine β-defensin (pBD) gene-like sequences, including 17 unreported pBDs in the porcine genome. Comparative analysis of β-defensin genes in the pig genome with those in human and cattle genomes showed structural conservation of β-defensin syntenic regions among these species.

【 授权许可】

Unknown   
© 2012 Choi et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.