Identification of key lncRNAs in colorectal cancer progression based on associated protein–protein interaction analysis

World Journal of Surgical Oncology

Research

Jiajing Yu¹  Shengjie Feng¹  Yusheng Guo¹  Haifeng Zhu²  Haishan Zhu² 

[1] Huashan Hospital, Fudan University, Shanghai, China;The First Hospital of ZhaoQing, Guangdong, China;

关键词: Long non-coding RNA;    Colorectal cancer;    Protein–protein interaction analysis;    Expression profiling;   

DOI  :  10.1186/s12957-017-1211-7

 received in 2017-04-08, accepted in 2017-07-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundColorectal cancer (CRC) was one of the most commonly diagnosed malignancies. The molecular mechanisms involved in the progression of CRC remain unclear. Accumulating evidences showed that long noncoding RNAs (lncRNAs) played key roles in tumorigenesis, cancer progression, and metastasis. Therefore, we aimed to explore the roles of lncRNAs in the progression of CRC.MethodsIn this study, we aimed to identify differentially expressed lncRNAs and messenger RNAs (mRNAs) in CRC by analyzing a cohort of previously published datasets: GSE64857. GO and KEGG pathway analyses were applied to give us insight in the functions of those lncRNAs and mRNAs in CRC.ResultsTotally, 46 lncRNAs were identified as differentially expressed between stage II and stage III CRC for the first time screening by microarray. GO and KEGG pathway analyses showed that differentially expressed lncRNAs were involved in regulating signal transduction, cell adhesion, cell differentiation, focal adhesion, and cell adhesion molecules.ConclusionsWe found three lncRNAs (LOC100129973, PGM5-AS1, and TTTY10) widely co-expressed with differentially expressed mRNAs. We also constructed lncRNA-associated PPI in CRC and found that these lncRNAs may be associated with CRC progression. Moreover, we found that high PGM5-AS1 expression levels were associated with worse overall survival in CRC cancer. We believe that this study would provide novel potential therapeutic and prognostic targets for CRC.

【 授权许可】

CC BY   
© The Author(s). 2017