A first assessment of the genetic diversity of Mycobacterium tuberculosis complex in Cambodia

BMC Infectious Diseases

Research Article

Bertrand Guillard¹  Seiha Heng¹  Stéphanie Le Moullec²  Jian Zhang²  Guislaine Refregier²  Christophe Sola³  Brigitte Gicquel⁴ 

[1] Institut Pasteur du Cambodge, Phnom-Penh, BP 983, 5 boulevard Monivong, Phnom-Penh, Cambodia;Institut de Génétique et Microbiologie, UMR8621 CNRS-Université Paris-Sud 11, UniverSud, Infection Genetics Emerging Pathogens Evolution (IGEPE) Team, Bât. 400, F-91405, Orsay-Cedex, France;Institut de Génétique et Microbiologie, UMR8621 CNRS-Université Paris-Sud 11, UniverSud, Infection Genetics Emerging Pathogens Evolution (IGEPE) Team, Bât. 400, F-91405, Orsay-Cedex, France;Unité de Génétique Mycobactérienne, Institut Pasteur, Paris, France;Unité de Génétique Mycobactérienne, Institut Pasteur, Paris, France;

关键词: Clinical Isolate;    Mycobacterium Tuberculosis Complex;    Mycobacterium Tuberculosis Complex;    Beijing Family;    Mycobacterial Intersperse Repetitive Unit;   

DOI  :  10.1186/1471-2334-11-42

 received in 2010-10-08, accepted in 2011-02-07,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCambodia is among the 22 high-burden TB countries, and has one of the highest rates of TB in South-East Asia. This study aimed to describe the genetic diversity among clinical Mycobacterium tuberculosis complex (MTC) isolates collected in Cambodia and to relate these findings to genetic diversity data from neighboring countries.MethodsWe characterized by 24 VNTR loci genotyping and spoligotyping 105 Mycobacterium tuberculosis clinical isolates collected between 2007 and 2008 in the region of Phnom-Penh, Cambodia, enriched in multidrug-resistant (MDR) isolates (n = 33).ResultsClassical spoligotyping confirmed that the East-African Indian (EAI) lineage is highly prevalent in this area (60%-68% respectively in whole sample and among non-MDR isolates). Beijing lineage is also largely represented (30% in whole sample, 21% among non-MDR isolates, OR = 4.51, CI95% [1.77, 11.51]) whereas CAS lineage was absent. The 24 loci MIRU-VNTR typing scheme distinguished 90 patterns with only 13 multi-isolates clusters covering 28 isolates. The clustering of EAI strains could be achieved with only 8 VNTR combined with spoligotyping, which could serve as a performing, easy and cheap genotyping standard for this family. Extended spoligotyping suggested relatedness of some unclassified "T1 ancestors" or "Manu" isolates with modern strains and provided finer resolution.ConclusionsThe genetic diversity of MTC in Cambodia is driven by the EAI and the Beijing families. We validate the usefulness of the extended spoligotyping format in combination with 8 VNTR for EAI isolates in this region.

【 授权许可】

Unknown   
© Zhang et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.