Kinetic modeling of Shewanella baltica KB30 growth on different substrates through respirometry

Microbial Cell Factories

Research

José Manuel Poyatos¹  Juan Carlos Leyva-Díaz²  Susanna Gorrasi³  Paolo Barghini³  Massimiliano Fenice⁴ 

[1] Department of Civil Engineering, University of Granada, 18071, Granada, Spain;Institute for Water Research, University of Granada, 18071, Granada, Spain;Department of Civil Engineering, University of Granada, 18071, Granada, Spain;Institute for Water Research, University of Granada, 18071, Granada, Spain;Department of Ecological and Biological Sciences, University of Tuscia, Largo Università snc, 01100, Viterbo, Italy;Department of Ecological and Biological Sciences, University of Tuscia, Largo Università snc, 01100, Viterbo, Italy;Department of Ecological and Biological Sciences, University of Tuscia, Largo Università snc, 01100, Viterbo, Italy;Laboratory of Applied Marine Microbiology, ConISMa, University of Tuscia, 01100, Viterbo, Italy;

关键词: Carbon utilization;    Kinetics;    Modeling;    Shewanella baltica;   

DOI  :  10.1186/s12934-017-0805-7

 received in 2017-07-14, accepted in 2017-10-31,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundShewanella baltica KB30 was isolated from seawater collected in Kandalaksha Bay, White Sea (Russia). This strain is known for its ability to grow on a pool of different substrates, including carbohydrates, carboxylic and amino acids, and lipids. However, no data are available on its metabolic efficiency in relation to the use of different carbon sources typologies. This work represents the first attempt to characterize S. baltica by its heterotrophic kinetic performance.ResultsGrowth and substrate consumption, during the biodegradation of sodium acetate, glucose, tween 80 and peptone, were analyzed through a respirometric method. To find the model best fitting the experimental data and to obtain the kinetic parameters, the equations of Monod, Moser, Contois and Tessier were applied. The kinetic behavior of S. baltica was fitted to Monod model for sodium acetate and tween 80, while it was adjusted to Contois model for glucose and peptone. In this regard, peptone was consumed faster than the other substrates, as indicated by the highest values of substrate degradation rate, which exceeded 60 mg O2 L−1 h−1.ConclusionsProteolytic metabolism was favored than lipidic and glucidic metabolism, which could contribute much more to mineralization and recycling of proteins than lipids and carbohydrates.

【 授权许可】

CC BY   
© The Author(s) 2017

【 图 表 】

Fig. 1