Multidimensional optimization for accelerating light-powered biocatalysis in Rhodopseudomonas palustris

Biotechnology for Biofuels and Bioproducts

Research

Wenchang Meng¹  Yuting He¹  Yuhui Chen¹  Mingyu Shao¹  Yang Zhang²  Jifeng Yuan² 

[1] State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, 361102, Fujian, China;State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, 361102, Fujian, China;Shenzhen Research Institute of Xiamen University, 518057, Shenzhen, China;

关键词: Light-driven biocatalysis;    Cofactor regeneration;    Lignocellulose upcycling;    Isoprenol utilization pathway;    Rhodopseudomonas palustris;   

DOI  :  10.1186/s13068-023-02410-3

 received in 2023-08-02, accepted in 2023-10-12,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundWhole-cell biocatalysis has been exploited to convert a variety of substrates into high-value bulk or chiral fine chemicals. However, the traditional whole-cell biocatalysis typically utilizes the heterotrophic microbes as the biocatalyst, which requires carbohydrates to power the cofactor (ATP, NAD (P)H) regeneration.ResultsIn this study, we sought to harness purple non-sulfur photosynthetic bacterium (PNSB) as the biocatalyst to achieve light-driven cofactor regeneration for cascade biocatalysis. We substantially improved the performance of Rhodopseudomonas palustris-based biocatalysis using a highly active and conditional expression system, blocking the side-reactions, controlling the feeding strategy, and attenuating the light shading effect. Under light-anaerobic conditions, we found that 50 mM ferulic acid could be completely converted to vanillyl alcohol using the recombinant strain with 100% efficiency, and > 99.9% conversion of 50 mM p-coumaric acid to p-hydroxybenzyl alcohol was similarly achieved. Moreover, we examined the isoprenol utilization pathway for pinene synthesis and 92% conversion of 30 mM isoprenol to pinene was obtained.ConclusionsTaken together, these results suggested that R. palustris could be a promising host for light-powered biotransformation, which offers an efficient approach for synthesizing value-added chemicals in a green and sustainable manner.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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