Free fatty acids profile among lean, overweight and obese non-alcoholic fatty liver disease patients: a case – control study

Lipids in Health and Disease

Research

Chunlong Li¹  Shanshan Du²  Yucun Niu²  Yang Chen²  Tianqi Zi²  Akinkunmi Paul Okekunle²  Rennan Feng²  Yanchuan Li²  Chao Luo³ 

[1] Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, 150081, Harbin, Heilongjiang Province, China;Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, 157 Baojian Street, Nangang District, 150081, Harbin, Heilongjiang Province, China;STD & AIDS Center, Harbin Center for Disease Control and Prevention, 150056, Harbin, Heilongjiang Province, China;

关键词: FFA;    Body weight;    NAFLD;    GC-MS;    Myristic acid;    Palmitoleic acid;   

DOI  :  10.1186/s12944-017-0551-1

 received in 2017-03-31, accepted in 2017-08-15,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundNon-alcoholic fatty liver disease (NAFLD) given its association with obesity and diabetes may perhaps exert distinct free fatty acids (FFA) pattern, but the understanding of this phenomenon is limited. To this effect, we evaluated FFA profiles among healthy subjects and NAFLD patients stratified by body weight, to identify FFA valuable for early diagnosis of NAFLD.MethodsSerum FFA profiles of healthy and NAFLD (lean, overweight and obese) subjects was determined using gas chromatography–mass spectrometry (GC–MS) and distinctions in FFA patterns were evaluated using one-way ANOVA while Receiver operating characteristics (ROC) and logistic regression models were used to explore FFA significant for diagnosing NAFLD.ResultsNAFLD patients presented significantly higher (P < 0.05) serum FFA profiles compared to healthy controls (HC). While total FFA profiles were insignificantly different between lean (2093.33 ± 558.11 μg/ml) and overweight (2420.81 ± 555.18 μg/ml) NAFLD patients, obese NAFLD (2739.01 ± 810.35 μg/ml) presented most significantly elevated (P < 0.05) total FFA profiles compared with HC. Of the four FFA; myristic acid (14:0), palmitoleic acid (16:1), γ-linolenic acid (γ-18:3) and cis-7,10,13,16,19-docosapentaenoic acid (22:5), selected in ROC analysis given their high Youden’s index and AUC, only 14:0; 5.58(1.37, 22.76) and 16:1; 4.36(1.34, 14.13) had statistical significant odd ratios.ConclusionOur findings suggest 14:0 and 16:1 are promising for early diagnosis of NAFLD.

【 授权许可】

CC BY   
© The Author(s). 2017