Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina

BMC Veterinary Research

Research Article

José L Konrad¹  Gustavo Crudelli¹  Gustavo Combessies²  Axel Mauroy³  Etienne Thiry³  Osvaldo Zabal⁴  Julian Diodati⁴  Viviana Parreño⁴  Patricia M Lomonaco⁴  Daniela Rodriguez⁴  María I Craig⁵  Sonia A Romera⁵  Silvina S Maidana⁵ 

[1] Facultad de Veterinaria, Universidad Nacional del Nordeste, 25 de Mayo 868, 3400, Corrientes, Argentina;Laboratorio Azul, Av., 25 de Mayo, 479/485 (7300), Azul, Buenos Aires, Argentina;Veterinary Virology and Animal Viral Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonster, 20, B-4000, B43b, Liège, Belgium;de Virología, Centro de Investigaciones en Ciencias veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), N, Repetto y Los Reseros S/N, CC25 (B1712WAA), Buenos Aires, Castelar, Argentina;de Virología, Centro de Investigaciones en Ciencias veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), N, Repetto y Los Reseros S/N, CC25 (B1712WAA), Buenos Aires, Castelar, Argentina;Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), 1917 (C1033AAJ), Rivadavia, Ciudad Autónoma de Buenos Aires, Argentina;

关键词: Bovine Viral Diarrhea Virus;    Water Buffalo;    Vaginal Swab;    Bovine Respiratory Syncytial Virus;    Feedlot Cattle;   

DOI  :  10.1186/1746-6148-8-83

 received in 2012-03-09, accepted in 2012-06-07,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundParainfluenza virus type 3 (PIV3) was isolated from dairy buffaloes (Bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions.ResultsExamination of nasal and vaginal swabs collected from 12 diseased buffaloes led to the isolation of three paramyxovirus isolates from two animals. Antigenic, morphological and biological characteristics of these three isolates were essentially similar to those of members of the Paramyxoviridae family. Antigenic analysis by direct immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).ConclusionsThis is the first characterization of BPIV3 in water buffalo.According to the samples analyzed, in Argentina, the genotype B was found in buffalo and the genotypes A and C were found in cattle.

【 授权许可】

Unknown   
© Maidana et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.