Co-occurrence of resistance to different antibiotics among aquatic bacteria

BMC Microbiology

Research Article

Arvi Jõers¹  Veljo Kisand¹  Tanel Tenson¹  Veiko Voolaid¹ 

[1] University of Tartu Institute of Technology, Nooruse St 1, 50411, Tartu, Estonia;

关键词: Multiresistance;    Natural environment;    Fresh water;   

DOI  :  10.1186/1471-2180-12-225

 received in 2012-03-08, accepted in 2012-09-24,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAntibiotic resistance is not confined to pathogens, but is also widespread in various natural environments. In nature the microbes producing antibiotic compounds have been around for millions of years. Heavy use of antibiotics in medicine and veterinary practice may lead to the accumulation of resistance genes in microbial populations, followed by a rise in multiresistant bacteria.ResultsTo test the extent of resistance among aquatic bacteria, we have collected 760 isolates resistant to at least one antibiotic. The phylogeny of the isolates covers a wide range of Proteobacteria, Actinobacteria and Bacteroidetes. In order to determine the extent of multiresistance, the isolates were tested on six antibiotics. As the growth rate of the different bacteria was highly variable, the classical medical resistance tests could not be used, and an alternative method considering the full growth curve was developed. In general, the overall resistances to different antibiotics could be explained by random, independent distribution. An exception to this was the resistances against tetracycline and chloramphenicol, which tended to occur in pairs.ConclusionsWe conclude that there is no massive spread of multiresistance determinants in the studied environment, although some specific cases can be found, awaiting for molecular characterization of the resistance mechanisms.

【 授权许可】

Unknown   
© Voolaid et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.