Cholesteryl Ester Transfer Protein (CETP) expression does not affect glucose homeostasis and insulin secretion: studies in human CETP transgenic mice

Lipids in Health and Disease

Research

Emerielle C. Vanzela¹  Helena C F Oliveira¹  Helena F. Raposo¹  Jairo A. Berti² 

[1] Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Unicamp - Cidade Universitária Zeferino Vaz. Rua Monteiro Lobato, 255, CEP 13083-862, Campinas, SP, Brazil;Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Unicamp - Cidade Universitária Zeferino Vaz. Rua Monteiro Lobato, 255, CEP 13083-862, Campinas, SP, Brazil;Present address: Department of Physiological Science, State University of Maringa, Maringa, PR, Brazil;

关键词: Cholesteryl ester transfer protein (CETP);    Glucose homeostasis;    Insulin sensitivity;    Glucose uptake;   

DOI  :  10.1186/s12944-016-0179-6

 received in 2015-05-05, accepted in 2016-01-07,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCholesteryl ester transfer protein (CETP) is a plasma protein that mediates the exchange of triglycerides for esterified cholesterol between HDL and apoB-lipoproteins. Previous studies suggest that CETP may modify glucose metabolism in patients or cultured cells. In this study, we tested if stable CETP expression would impair glucose metabolism.MethodsWe used human CETP transgenic mice and non-transgenic littermate controls (NTg), fed with control or high fat diet, as well as in dyslipidemic background and aging conditions. Assays included glucose and insulin tolerance tests, isolated islets insulin secretion, tissue glucose uptake and adipose tissue GLUT mRNA expression.ResultsCETP expression did not modify glucose or insulin tolerance in all tested conditions such as chow and high fat diet, adult and aged mice, normo and dyslipidemic backgrounds. Fasting and fed state plasma levels of insulin were not differ in CETP and NTg mice. Direct measurements of isolated pancreatic islet insulin secretion rates induced by glucose (11, 16.7 or 22 mM), KCl (40 mM), and leucine (10 mM) were similar in NTg and CETP mice, indicating that CETP expression did not affect β-cell function in vivo and ex vivo. Glucose uptake by insulin target tissues, measured in vivo using 3H-2-deoxyglucose, showed that CETP expression had no effect on the glucose uptake in liver, muscle, perigonadal, perirenal, subcutaneous and brown adipose tissues. Accordingly, GLUT1 and GLUT4 mRNA in adipose tissue were not affected by CETP.ConclusionsIn summary, by comparing the in vivo all-or-nothing CETP expressing mouse models, we demonstrated that CETP per se has no impact on the glucose tolerance and tissue uptake, global insulin sensitivity and beta cell insulin secretion rates.

【 授权许可】

CC BY   
© Raposo et al. 2016