Transcriptomic data from panarthropods shed new light on the evolution of insulator binding proteins in insects

BMC Genomics

Research Article

Xin Zhou¹  Bernhard Misof²  Oliver Niehuis²  Thomas Pauli²  Alexander Donath²  Christoph Mayer²  Malte Petersen²  Karen Meusemann³  Shanlin Liu⁴  Lars Hering⁵  Georg Mayer⁵  Daniel Dowling⁶  Lars Podsiadlowski⁷  Peter Heger⁸  Thomas Wiehe⁸  Lucia Vedder⁹  Ralph S. Peters^1,10 

[1] Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, 100193, Beijing, China;College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China;Center of Molecular Biodiversity Research, Zoological Research Museum Alexander Koenig, Adenauerallee 160, 51113, Bonn, Germany;Center of Molecular Biodiversity Research, Zoological Research Museum Alexander Koenig, Adenauerallee 160, 51113, Bonn, Germany;Department for Evolutionary Biology and Ecology (Institut for Biology I, Zoology), University of Freiburg, Hauptstr. 1, 79104, Freiburg, Germany;Australian National Insect Collection, CSIRO National Research Collections Australia, Clunies Ross Street, 2601, Acton, ACT, Australia;China National GeneBank-Shenzhen, BGI-Shenzhen, 518083, Shenzhen, Guangdong Province, China;Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark;Department of Zoology, University of Kassel, Heinrich-Plett-Str. 40, 34132, Kassel, Germany;Johannes Gutenberg University Mainz, Institute of Molecular Biology (IMB), Ackermannweg 4, 55128, Mainz, Germany;University of Bonn, Institute of Evolutionary Biology and Ecology, An der Immenburg 1, 53121, Bonn, Germany;University of Cologne, Cologne Biocenter, Institute for Genetics, Zülpicher Straße 47a, 50674, Köln, Germany;University of Tübingen, Geschwister-Scholl-Platz, 72074, Tübingen, Germany;Zoological Research Museum Alexander Koenig, Arthropod Department, Adenauerallee 160, 53113, Bonn, Germany;

关键词: Insulator binding proteins;    Comparative transcriptomic analyses;    Gene evolution;    Arthropod evolution;   

DOI  :  10.1186/s12864-016-3205-1

 received in 2016-06-07, accepted in 2016-10-25,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBody plan development in multi-cellular organisms is largely determined by homeotic genes. Expression of homeotic genes, in turn, is partially regulated by insulator binding proteins (IBPs). While only a few enhancer blocking IBPs have been identified in vertebrates, the common fruit fly Drosophila melanogaster harbors at least twelve different enhancer blocking IBPs. We screened recently compiled insect transcriptomes from the 1KITE project and genomic and transcriptomic data from public databases, aiming to trace the origin of IBPs in insects and other arthropods.ResultsOur study shows that the last common ancestor of insects (Hexapoda) already possessed a substantial number of IBPs. Specifically, of the known twelve insect IBPs, at least three (i.e., CP190, Su(Hw), and CTCF) already existed prior to the evolution of insects. Furthermore we found GAF orthologs in early branching insect orders, including Zygentoma (silverfish and firebrats) and Diplura (two-pronged bristletails). Mod(mdg4) is most likely a derived feature of Neoptera, while Pita is likely an evolutionary novelty of holometabolous insects. Zw5 appears to be restricted to schizophoran flies, whereas BEAF-32, ZIPIC and the Elba complex, are probably unique to the genus Drosophila. Selection models indicate that insect IBPs evolved under neutral or purifying selection.ConclusionsOur results suggest that a substantial number of IBPs either pre-date the evolution of insects or evolved early during insect evolution. This suggests an evolutionary history of insulator binding proteins in insects different to that previously thought. Moreover, our study demonstrates the versatility of the 1KITE transcriptomic data for comparative analyses in insects and other arthropods.

【 授权许可】

CC BY   
© The Author(s). 2016