BMC Infectious Diseases | |
Identification and antimicrobial resistance patterns of bacterial enteropathogens from children aged 0–59 months at the University Teaching Hospital, Lusaka, Zambia: a prospective cross sectional study | |
Research Article | |
Geoffrey Kwenda1 John B. Muma2 Harriet Chiyangi3 Mecky I. Matee4 Sydney Malama5 Joel Manyahi5 Ahmed Abade6 | |
[1] Department of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka, Zambia;Department of Disease Control, School of Veterinary, University of Zambia, Lusaka, Zambia;Department of Epidemiology and Biostatistics, School of Public Health and Social Sciences, Muhimbili University of Health and Allied Science, Dar es Salaam, Tanzania;Tanzania Field Epidemiology and Laboratory Management Program, Ministry of Health, Dar es Salaam, Tanzania;Department of Microbiology and Immunology, School of Medicine, Muhimbili University of Health and Allied Science, Dar es Salaam, Tanzania;Health Promotions Research Program, Institute of Economic and Social Research, University of Zambia, Lusaka, Zambia;Tanzania Field Epidemiology and Laboratory Management Program, Ministry of Health, Dar es Salaam, Tanzania; | |
关键词: Bacteria; Diarrhea; Children; Antimicrobial; Zambia; | |
DOI : 10.1186/s12879-017-2232-0 | |
received in 2016-11-21, accepted in 2017-01-31, 发布年份 2017 | |
来源: Springer | |
【 摘 要 】
BackgroundBacterial diarrhoeal disease is among the most common causes of mortality and morbidity in children 0–59 months at the University Teaching Hospital in Lusaka, Zambia. However, most cases are treated empirically without the knowledge of aetiological agents or antimicrobial susceptibility patterns. The aim of this study was, therefore, to identify bacterial causes of diarrhoea and determine their antimicrobial susceptibility patterns in stool specimens obtained from the children at the hospital.MethodsThis hospital-based cross-sectional study involved children aged 0–59 months presenting with diarrhoea at paediatrics wards at the University Teaching Hospital in Lusaka, Zambia, from January to May 2016. Stool samples were cultured on standard media for enteropathogenic bacteria, and identified further by biochemical tests. Multiplex polymerase chain reaction was used for characterization of diarrhoeagenic Escherichia coli strains. Antimicrobial susceptibility testing was performed on antibiotics that are commonly prescribed at the hospital using the Kirby-Bauer disc diffusion method, which was performed using the Clinical Laboratory Standards International guidelines.ResultsOf the 271 stool samples analysed Vibrio cholerae 01 subtype and Ogawa serotype was the most commonly detected pathogen (40.8%), followed by Salmonella species (25.5%), diarrhoeagenic Escherichia coli (18%), Shigella species (14.4%) and Campylobacter species (3.5%). The majority of the bacterial pathogens were resistant to two or more drugs tested, with ampicillin and co-trimoxazole being the most ineffective drugs. All diarrhoeagenic Escherichia coli isolates were extended spectrum β-lactamase producers.ConclusionFive different groups of bacterial pathogens were isolated from the stool specimens, and the majority of these organisms were multidrug resistant. These data calls for urgent revision of the current empiric treatment of diarrhoea in children using ampicillin and co-trimoxazole, and emphasizes the need for continuous antimicrobial surveillance as well as the implementation of prevention programmes for childhood diarrhoea.
【 授权许可】
CC BY
© The Author(s). 2017
【 预 览 】
Files | Size | Format | View |
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RO202311104829243ZK.pdf | 481KB | download |
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