Genetic diversity among eight Dendrolimus species in Eurasia (Lepidoptera: Lasiocampidae) inferred from mitochondrial COI and COII, and nuclear ITS2 markers

BMC Genetics

Research

Victor Fet¹  Alexander Blinov²  Kirill Ustyantsev²  Alexander Kononov²  Victor C. Mastro³  Baode Wang³  Yuri Baranchikov⁴ 

[1] Department of Biological Sciences, Marshall University, 1601 5th Avenue, 25755, Huntington, WV, USA;Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Science, 10 Prospekt Lavrentyeva, 630090, Novosibirsk, Russia;USDA-APHIS-PPQ CPHST, Otis Laboratory, Building 1398, Otis Air National Guard Base, 02542, Buzzards Bay, MA, USA;V.N. Sukachev Institute of Forest, the Siberian Branch of the Russian Academy of Science, 50/28 Akademgorodok, 660036, Krasnoyarsk, Russia;

关键词: Dendrolimus;    Phylogeny;    Pests;    Interspecific hybridization;    Divergence;   

DOI  :  10.1186/s12863-016-0463-5

来源: Springer

【 摘 要 】

BackgroundMoths of genus Dendrolimus (Lepidoptera: Lasiocampidae) are among the major pests of coniferous forests worldwide. Taxonomy and nomenclature of this genus are not entirely established, and there are many species with a controversial taxonomic position. We present a comparative evolutionary analysis of the most economically important Dendrolimus species in Eurasia.ResultsOur analysis was based on the nucleotide sequences of COI and COII mitochondrial genes and ITS2 spacer of nuclear ribosomal genes. All known sequences were extracted from GenBank. Additional 112 new sequences were identified for 28 specimens of D. sibiricus, D. pini, and D. superans from five regions of Siberia and the Russian Far East to be able to compare the disparate data from all previous studies. In total, 528 sequences were used in phylogenetic analysis. Two clusters of closely related species in Dendrolimus were found. The first cluster includes D. pini, D. sibiricus, and D. superans; and the second, D. spectabilis, D. punctatus, and D. tabulaeformis. Species D. houi and D. kikuchii appear to be the most basal in the genus.ConclusionGenetic difference among the second cluster species is very low in contrast to the first cluster species. Phylogenetic position D. tabulaeformis as a subspecies was supported. It was found that D. sibiricus recently separated from D. superans. Integration of D. sibiricus mitochondrial DNA sequences and the spread of this species to the west of Eurasia have been established as the cause of the unjustified allocation of a new species: D. kilmez. Our study further clarifies taxonomic problems in the genus and gives more complete information on the genetic structure of D. pini, D. sibiricus, and D. superans.

【 授权许可】

CC BY   
© The Author(s). 2016