Establishment of high reciprocal connectivity between clonal cortical neurons is regulated by the Dnmt3b DNA methyltransferase and clustered protocadherins

BMC Biology

Research Article

Takahiro Hirabayashi¹  Sonoko Hasegawa¹  Takeshi Yagi¹  Takashi Kitsukawa¹  Teruyoshi Hirayama¹  Shunsuke Toyoda¹  Masumi Hirabayashi²  Ryosuke Kaneko³  Megumi Kato-Itoh⁴  Toshihiro Kobayashi⁴  Hiromitsu Nakauchi⁵  Atsushi Okayama⁶  Makoto Sanbo⁷  Toshio Miyashita⁸  Etsuko Tarusawa⁹  Yumiko Yoshimura^1,10 

[1] AMED-CREST, AMED, 1-3 Yamadaoka, 565-0871, Suita, Osaka, Japan;KOKORO-Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, 565-0871, Suita, Osaka, Japan;AMED-CREST, AMED, 1-3 Yamadaoka, 565-0871, Suita, Osaka, Japan;National Institute for Physiological Sciences, Section of Mammalian Transgenesis, Center for Genetic Analysis of Behavior, 444-8787, Okazaki, Aichi, Japan;Department of Physiological Sciences, The Graduate University for Advanced Studies (SOKENDAI), 444-8585, Okazaki, Aichi, Japan;Bioresource Center, Gunma University Graduate School of Medicine, 371-8511, Maebashi, Japan;Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, 108-8639, Tokyo, Japan;Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, 108-8639, Tokyo, Japan;Department of Genetics, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 291 Campus Drive, Li Ka Shing Building, 94305-5101, Stanford, CA, USA;KOKORO-Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, 565-0871, Suita, Osaka, Japan;National Institute for Physiological Sciences, Section of Mammalian Transgenesis, Center for Genetic Analysis of Behavior, 444-8787, Okazaki, Aichi, Japan;Section of Visual Information Processing, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 444-8585, Okazaki, Aichi, Japan;Section of Visual Information Processing, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 444-8585, Okazaki, Aichi, Japan;AMED-CREST, AMED, 1-3 Yamadaoka, 565-0871, Suita, Osaka, Japan;Section of Visual Information Processing, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 444-8585, Okazaki, Aichi, Japan;Department of Physiological Sciences, The Graduate University for Advanced Studies (SOKENDAI), 444-8585, Okazaki, Aichi, Japan;

关键词: Barrel cortex;    Spiny stellate cell;    Clonal neuron;    iPS cells;    Connection specificity;    Reciprocity;    Dnmt3b;    Clustered protocadherin;   

DOI  :  10.1186/s12915-016-0326-6

 received in 2016-09-27, accepted in 2016-11-09,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe specificity of synaptic connections is fundamental for proper neural circuit function. Specific neuronal connections that underlie information processing in the sensory cortex are initially established without sensory experiences to a considerable extent, and then the connections are individually refined through sensory experiences. Excitatory neurons arising from the same single progenitor cell are preferentially connected in the postnatal cortex, suggesting that cell lineage contributes to the initial wiring of neurons. However, the postnatal developmental process of lineage-dependent connection specificity is not known, nor how clonal neurons, which are derived from the same neural stem cell, are stamped with the identity of their common neural stem cell and guided to form synaptic connections.ResultsWe show that cortical excitatory neurons that arise from the same neural stem cell and reside within the same layer preferentially establish reciprocal synaptic connections in the mouse barrel cortex. We observed a transient increase in synaptic connections between clonal but not nonclonal neuron pairs during postnatal development, followed by selective stabilization of the reciprocal connections between clonal neuron pairs. Furthermore, we demonstrate that selective stabilization of the reciprocal connections between clonal neuron pairs is impaired by the deficiency of DNA methyltransferase 3b (Dnmt3b), which determines DNA-methylation patterns of genes in stem cells during early corticogenesis. Dnmt3b regulates the postnatal expression of clustered protocadherin (cPcdh) isoforms, a family of adhesion molecules. We found that cPcdh deficiency in clonal neuron pairs impairs the whole process of the formation and stabilization of connections to establish lineage-specific connection reciprocity.ConclusionsOur results demonstrate that local, reciprocal neural connections are selectively formed and retained between clonal neurons in layer 4 of the barrel cortex during postnatal development, and that Dnmt3b and cPcdhs are required for the establishment of lineage-specific reciprocal connections. These findings indicate that lineage-specific connection reciprocity is predetermined by Dnmt3b during embryonic development, and that the cPcdhs contribute to postnatal cortical neuron identification to guide lineage-dependent synaptic connections in the neocortex.

【 授权许可】

CC BY   
© Yoshimura et al. 2016