SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis

BMC Plant Biology

Research Article

Shengchun Zhang¹  Jianbin Lai¹  Yaqin Wang¹  Chengwei Yang¹  Jinju Du¹  Dongke Yuan¹  Yiyang Liu¹  Songfeng Shi¹  Panglian Xu¹  Ming Liu² 

[1] Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, 510631, Guangzhou, China;Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, 510631, Guangzhou, China;Vegetable Research Institute Guangdong Academy of Agriculture Sciences, 510640, Guangzhou, Guangdong, China;

关键词: AtMMS21;    SUMOylation;    Gametophyte development;    Meiosis;    Arabidopsis thaliana;   

DOI  :  10.1186/1471-2229-14-153

 received in 2013-12-01, accepted in 2014-05-28,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMMS21 is a SUMO E3 ligase that is conserved in eukaryotes, and has previously been shown to be required for DNA repair and maintenance of chromosome integrity. Loss of the Arabidopsis MMS21 causes defective meristems and dwarf phenotypes.ResultsHere, we show a role for AtMMS21 during gametophyte development. AtMMS21 deficient plants are semisterile with shorter mature siliques and abortive seeds. The mms21-1 mutant shows reduced pollen number, and viability, and germination and abnormal pollen tube growth. Embryo sac development is also compromised in the mutant. During meiosis, chromosome mis-segregation and fragmentation is observed, and the products of meiosis are frequently dyads or irregular tetrads. Several transcripts for meiotic genes related to chromosome maintenance and behavior are altered. Moreover, accumulation of SUMO-protein conjugates in the mms21-1 pollen grains is distinct from that in wild-type.ConclusionsThus, these results suggest that AtMMS21 mediated SUMOylation may stabilize the expression and accumulation of meiotic proteins and affect gametophyte development.

【 授权许可】

Unknown   
© Liu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.