Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation

Environmental Health

Research

Irène Sari-Minodier¹  Hamzeh Al Zabadi²  Luc Ferrari³  Christophe Paris⁴  Denis Zmirou-Navier⁵  Aziz Tiberguent⁶  Marie-Aude Kerautret⁷ 

[1] Aix-Marseille University School of Medicine, EA 1784, 13005, Marseille, France;An-Najah National University, School of Medicine-Public Health Department, Nablus, Palestinian;INSERM U954, School of Medicine, 54505 Vandœuvre-lès-Nancy, France;Nancy University School of Medicine, 54505 Vandœuvre-lès-Nancy, France;INSERM U954, School of Medicine, 54505 Vandœuvre-lès-Nancy, France;Nancy University Faculty of Pharmacy, 54000, Nancy, France;INSERM U954, School of Medicine, 54505 Vandœuvre-lès-Nancy, France;Nancy University School of Medicine, 54505 Vandœuvre-lès-Nancy, France;INSERM U954, School of Medicine, 54505 Vandœuvre-lès-Nancy, France;Nancy University School of Medicine, 54505 Vandœuvre-lès-Nancy, France;EHESP School of Public Health-IRSET, 35000, Rennes, France;Occupational medicine service of the City of Paris, France;Paris City Hygiene laboratory, Paris, France;

关键词: HepG2 Cell;    Comet Assay;    Office Worker;    Micronucleus Assay;    Lifetime Cancer Risk;   

DOI  :  10.1186/1476-069X-10-23

 received in 2010-11-21, accepted in 2011-03-24,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSewage workers are exposed to multiple chemicals among which many are suspected genotoxicants. Therefore, they might incur DNA damage and oxidative stress. We aimed to explore integrated urinary biomarkers, assessing the overall urine genotoxicity by in vitro comet and micronucleus assays and measuring urinary 8-oxo-2'-deoxyguanosine.MethodsDuring three consecutive working days, polycyclic aromatic hydrocarbons and volatile organic compounds were sampled in workplace air of 34 sewage and 30 office workers, as indicators of airborne exposure. The last day, subjects collected their 24 hours urine. Genotoxicity of urinary extracts was assessed by comet and micronucleus assays on a HepG2 cell line. Using competitive enzymatic immunoassay we evaluated the 24 hours urinary 8-oxo-2'-deoxyguanosine excretion. Benzo(a)pyrene toxicity equivalent factors and inhalation unit risk for Benzo(a)pyrene and benzene were used to give an estimate of cancer risk levels.ResultsWorkplace air concentrations of polycyclic aromatic hydrocarbons (e.g. 23.7 [range 2.4-104.6] ng.m-3 for fluoranthene) and volatile organic compounds (e.g. 19.1 ± 2.9 [standard error] μ.m-3 for benzene) were elevated in sewage compared to office workplaces (P < 0.01) and corresponded to an increased lifetime cancer risk. The urinary extracts of sewage workers showed higher genotoxicity (P < 0.001) than office workers.ConclusionsThe integrated and non-specific urinary biomarkers of exposure showed that sewage workers experience exposure to mixtures of genotoxicants in the workplace.

【 授权许可】

CC BY   
© Al Zabadi et al; licensee BioMed Central Ltd. 2011