Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury

Journal of Inflammation

Research

Gordon S Lynch¹  Kristy Swiderski¹  Chris van der Poel²  Jonathan D Schertzer³  Meron Wondemaghen⁴  James G Ryall⁵  Luc E Gosselin⁶ 

[1] Basic and Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, 3010, Victoria, Australia;Basic and Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, 3010, Victoria, Australia;Current: Department of Human Biosciences, Faculty of Health Sciences, La Trobe University, 3086, Bundoora, VIC, Australia;Basic and Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, 3010, Victoria, Australia;Current: Department of Human Biosciences, Faculty of Health Sciences, La Trobe University, 3086, Bundoora, VIC, Australia;Current: Faculty of Health Sciences, Dept. of Biochemistry and Biomedical Sciences, L8S 4K1, Hamilton ON, Canada;Basic and Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, 3010, Victoria, Australia;Current: Faculty of Arts, Monash University, 3800, Clayton, Victoria, Australia;Basic and Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, 3010, Victoria, Australia;Current: The Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health (NIH), Bethesda, MD, USA;Department of Exercise and Nutrition Sciences, University of Buffalo, 14214, Buffalo NY, USA;

关键词: Bupivacaine;    Extensor Digitorum Longus;    Post Injury;    Extensor Digitorum Longus Muscle;    Injured Muscle;   

DOI  :  10.1186/1476-9255-8-41

 received in 2011-09-16, accepted in 2011-12-29,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSome of the most serious consequences of normal ageing relate to its effects on skeletal muscle, particularly significant wasting and associated weakness, termed "sarcopenia". The underlying mechanisms of sarcopenia have yet to be elucidated completely but an altered muscle inflammatory response after injury is a likely contributing factor. In this study we investigated age-related changes in the expression of numerous inflammatory markers linked to successful muscle regeneration.MethodsRight extensor digitorum longus (EDL) muscles from young (3 month), adult (12 month) and old (24 month) male F344 rats were injected with bupivacaine hydrochloride to cause complete muscle fibre degeneration, then excised 12, 24, 36, and 72 hours later (n = 5/age group/time point). We used qRT-PCR to quantify the mRNA expression levels of the inflammatory markers TNFα, IFNγ, IL1, IL18, IL6, and CD18 as well as regenerative markers MyoD and myogenin.ResultsInflammatory markers were all increased significantly in all age groups after myotoxic injury. There was a trend for expression of inflammatory markers to be higher in uninjured muscles of old rats, especially at 72 hours post injury where the expression levels of several markers was significantly higher in old compared with young and adult rats. There was also a decrease in the expression of regenerative markers in old rats at 72 hours post injury.ConclusionOur findings identify a prolonged inflammatory signature in injured muscles from old compared with young and adult rats together with a blunted expression of key markers of regeneration in muscles of old rats. Importantly, our findings identify potential targets for future therapeutic strategies for improving the regenerative capacity of skeletal muscle during ageing.

【 授权许可】

Unknown   
© van der Poel et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.