The invasome of Salmonella Dublin as revealed by whole genome sequencing

BMC Infectious Diseases

Research Article

Manal Mohammed¹  Rene Hendriksen²  Pimlapas Leekitcharoenphon²  Simon Le Hello³ 

[1] Department of Biomedical Sciences, Faculty of Science and Technology, University of Westminster, London, UK;National Food Institute, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and Genomics and European Union Reference Laboratory for Antimicrobial Resistance, Technical University of Denmark, -2800, Kgs. Lyngby, DK, Denmark;Unité des Bactéries Pathogènes Entériques, Centre National de Référence des Salmonella, WHO Collaborating Centre for Reference and Research on Salmonella, Institut Pasteur, Paris, France;

关键词: Salmonella;    Virulence;    SPI-6;    SPI-19;    T6SS;    Vi antigen;    PagN;    Ggt;    Gifsy-2;   

DOI  :  10.1186/s12879-017-2628-x

 received in 2017-01-10, accepted in 2017-07-21,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSalmonella enterica serovar Dublin is a zoonotic infection that can be transmitted from cattle to humans through consumption of contaminated milk and milk products. Outbreaks of human infections by S. Dublin have been reported in several countries including high-income countries. A high proportion of S. Dublin cases in humans are associated with invasive disease and systemic illness. The genetic basis of virulence in S. Dublin is not well characterized.MethodsWhole genome sequencing was applied to a set of clinical invasive and non-invasive S. Dublin isolates from different countries in order to characterize the putative genetic determinants involved in the virulence and invasiveness of S. Dublin in humans.ResultsWe identified several virulence factors that form the bacterial invasome and may contribute to increasing bacterial virulence and pathogenicity including mainly Gifsy-2 prophage, two different type 6 secretion systems (T6SSs) harbored by Salmonella pathogenicity islands; SPI-6 and SPI-19 respectively and virulence genes; ggt and PagN. Although Vi antigen and the virulence plasmid have been reported previously to contribute to the virulence of S. Dublin we did not detect them in all invasive isolates indicating that they are not the main virulence determinants in S. Dublin.ConclusionSeveral virulence factors within the genome of S. Dublin might contribute to the ability of S. Dublin to invade humans’ blood but there were no genomic markers that differentiate invasive from non-invasive isolates suggesting that host immune response play a crucial role in the clinical outcome of S. Dublin infection.

【 授权许可】

CC BY   
© The Author(s). 2017