Magnesium sulphate attenuate remifentanil-induced postoperative hyperalgesia via regulating tyrosine phosphorylation of the NR2B subunit of the NMDA receptor in the spinal cord

BMC Anesthesiology

Research Article

Wendong Lin¹  Hai Lin¹  Guodong He¹  Jianping Yang²  Jiehao Sun³ 

[1] Department of Anesthesiology, 1st Affiliated Hospital of Wenzhou Medical University, Wenzhou, China;Department of Anesthesiology, 1st Affiliated hospital, Soochow University, 188#, Shizi Street, Gusu District, 215000, Suzhou, China;Department of Anesthesiology, 1st Affiliated hospital, Soochow University, 188#, Shizi Street, Gusu District, 215000, Suzhou, China;Department of Anesthesiology, 1st Affiliated Hospital of Wenzhou Medical University, Wenzhou, China;

关键词: Magnesium;    Remifentanil;    Hyperalgesia;    NMDA receptor;    NRB;   

DOI  :  10.1186/s12871-017-0325-3

 received in 2016-09-28, accepted in 2017-02-17,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundRemifentanil induced hyperalgesia (RIH) is characterized by stimulation evoked pain including allodynia and thermal hyperalgesia after remifentanil infusion. N-methyl-D-aspartate (NMDA) receptor was reported to be involved in the progress of RIH. We hypothesized that intrathecal MgSO4 could relieve hyperalgesia after remifentanil infusion via regulating phosphorylation of NMDA receptor NR2B subunit activity in this study.MethodsThirty two rats were randomly allocated into control group, model of RIH group, RIH plus 100ug MgSO4 group, RIH plus 300ug MgSO4 group. Mechanical and thermal hyperalgesia were tested at -24th h, 2nd h, 6th h, 24th h, 48th h after remifentanil infusion. Following sacrifice of rats after the last behavioral test, we performed the western blot to detect the expression of spinal phosphorylated NMDA receptor NR2B subunit (pNR2B) in the L4-L5 segments.ResultsIntrathecal MgSO4 (100, 300 μg) dose-dependently reduced thermal and mechanical hyperalgesia from 2 h to 48 h after remifentanil infusion. Remifentanil infusion remarkably stimulated the expression of pNR2B. Nevertheless, the increased amount of pNR2B by RIH was dose-dependently suppressed by intrathecal infusion of MgSO4 in rats.ConclusionsRemifentanil induced hyperalgesia/allodynia could be ameliorated by Mg-mediated blockade targeting the NR2B subunit in NMDA receptors.

【 授权许可】

CC BY   
© The Author(s). 2017