Stability of pro- and anti-inflammatory immune biomarkers for human cohort studies

Journal of Translational Medicine

Research

W. P. Stefura¹  C. Graham¹  P. Lopez¹  L. Lotoski²  A. B. Becker³  K. T. HayGlass³  R. Chooniedass⁴  A. D. Befus⁵ 

[1] Department of Immunology, University of Manitoba, Winnipeg, MB, Canada;Department of Immunology, University of Manitoba, Winnipeg, MB, Canada;Community Health and Epidemiology-‎Saskatchewan Population Health and Evaluation Research Unit, University of Saskatchewan, Saskatoon, SK, Canada;Department of Immunology, University of Manitoba, Winnipeg, MB, Canada;Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada;Children’s Hospital Research Institute of Manitoba, Winnipeg, MB, Canada;Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada;Division of Pulmonary Medicine, Department of Medicine, University of Alberta, Edmonton, AB, Canada;

关键词: Cohort;    Inflammation;    Human immunology;    Immune regulation;    Innate;    Allergy;    Autoimmunity;   

DOI  :  10.1186/s12967-017-1154-3

 received in 2016-11-24, accepted in 2017-02-21,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAlthough discovery research has identified the importance of dozens of pro- and anti-inflammatory immune mediators in the pathogenesis, maintenance, exacerbation and resolution of inflammatory diseases, most human cohort studies have incorporated few or no immunological intermediate phenotypes in their analyses. Significant hindrances have been (1) the limited panel of biomarkers known to be readily detected in healthy human populations and (2) the stability, hence utility, of such biomarkers to repeated analysis.MethodsThe frequency and stability of 14 plasma biomarkers linked to in vivo immune regulation of allergic and autoimmune inflammatory disorders was determined in 140 healthy pediatric and adult participants. The impact of initial and multiple subsequent freeze/thaw cycles on pro-inflammatory (CCL2, CXCL10, IL-18, TNFα, IL-6), anti-inflammatory (IL-10, sTNF-RII, IL-1Ra), acute phase proteins (CRP, PTX3) and other biomarkers (sST2, IL-1RAcP) was subsequently quantified.ResultsMultiple biomarkers capable of providing an innate immune signature of inflammation were readily detected directly ex vivo in healthy individuals. These biomarker levels were unaffected when comparing paired data sets from freshly obtained, never frozen plasma or serum and matched aliquots despite extensive freeze/thaw cycles. Neither age nor sex affected stability. Similarly, no quantitative differences were found following repetitive analysis of inflammatory biomarkers in culture samples obtained following in vitro stimulation with TLR and RLR ligands.ConclusionsA broad panel of in vivo and ex vivo cytokine, chemokine and acute phase protein biomarkers that have been linked to human chronic inflammatory disorders are readily detected in vivo and remain stable for analysis despite multiple freeze thaw cycles. These data provide the foundation and confidence for large scale analyses of panels of inflammatory biomarkers to provide better understanding of immunological mechanisms underlying health versus disease.

【 授权许可】

CC BY   
© The Author(s) 2017