Knockout of MIMP protein in lactobacillus plantarumlost its regulation of intestinal permeability on NCM460 epithelial cells through the zonulin pathway

BMC Gastroenterology

Research Article

Jianping Wang¹  Chao Tong¹  Meijin Huang¹  Xingwei Zhang¹  Liang Kang¹  Nanqi Huang¹  Chao Li¹  Zhihua Liu²  Huanlong Qin³  Mary Pat Moyer⁴ 

[1] Department of Colorectal Surgery, Gastrointestinal Institute of Sun Yat-Sen University, the Sixth Affiliated Hospital of Sun Yat-Sen University (Guangdong Gastrointestinal Hospital), 26 Yuancun Erheng Road, 510655, Guangzhou, Guangdong, China;Department of Colorectal Surgery, Gastrointestinal Institute of Sun Yat-Sen University, the Sixth Affiliated Hospital of Sun Yat-Sen University (Guangdong Gastrointestinal Hospital), 26 Yuancun Erheng Road, 510655, Guangzhou, Guangdong, China;Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 200233, Shanghai, China;Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 200233, Shanghai, China;INCELL Corporation, 78249, San Antonio, Texas, USA;

关键词: Lactobacillus plantarum;    Micro integral membrane protein;    Zonulin;    Intestinal permeability;   

DOI  :  10.1186/1471-230X-14-171

 received in 2013-10-14, accepted in 2014-09-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPrevious studies indicated that the micro integral membrane protein located within the media place of the integral membrane protein of Lactobacillus plantarum CGMCC 1258 had protective effects against the intestinal epithelial injury. In our study, we mean to establish micro integral membrane protein -knockout Lactobacillus plantarum (LPKM) to investigate the change of its protective effects and verify the role of micro integral membrane protein on protection of normal intestinal barrier function.MethodsBinding assay and intestinal permeability were performed to verify the protective effects of micro integral membrane protein on intestinal permeability in vitro and in vivo. Molecular mechanism was also determined as the zonulin pathway. Clinical data were also collected for further verification of relationship between zonulin level and postoperative septicemia.ResultsLPKM got decreased inhibition of EPEC adhesion to NCM460 cells. LPKM had lower ability to alleviate the decrease of intestinal permeability induced by enteropathogenic-e.coli, and prevent enteropathogenic-e.coli -induced increase of zonulin expression. Overexpression of zonulin lowered the intestinal permeability regulated by Lactobacillus plantarum. There was a positive correlation between zonulin level and postoperative septicemia. Therefore, micro integral membrane protein could be necessary for the protective effects of Lactobacillus plantarum on intestinal barrier.ConclusionMIMP might be a positive factor for Lactobacillus plantarum to protect the intestinal epithelial cells from injury, which could be related to the zonulin pathway.

【 授权许可】

CC BY   
© Liu et al.; licensee BioMed Central Ltd. 2014