Profiles of low complexity regions in Apicomplexa

BMC Evolutionary Biology

Research Article

David Fisher¹  Fabia U. Battistuzzi²  Sophia Chaudhry³  Matthew K. Spencer⁴  Kristan A. Schneider⁵  Ananias A. Escalante⁶ 

[1] David Eccles School of Business, University of Utah, Salt Lake City, UT, USA;Department of Biological Sciences, Oakland University, Rochester, MI, USA;Department of Biological Sciences, Oakland University, Rochester, MI, USA;Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, USA;Department of Geology and Physics, Lake Superior State University, Sault Ste. Marie, MI, USA;Department of MNI, University of Applied Sciences Mittweida, Mittweida, Germany;Institute for Genomics and Evolutionary Medicine, Temple University, Philadelphia, PA, USA;

关键词: Low complexity regions;    Apicomplexa;    Repetitive regions;    Homopolymers;    Complexity threshold;    Plasmodium falciparum;    Composition bias;   

DOI  :  10.1186/s12862-016-0625-0

 received in 2015-09-12, accepted in 2016-02-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLow complexity regions (LCRs) are a ubiquitous feature in genomes and yet their evolutionary history and functional roles are unclear. Previous studies have shown contrasting evidence in favor of both neutral and selective mechanisms of evolution for different sets of LCRs suggesting that modes of identification of these regions may play a role in our ability to discern their evolutionary history. To further investigate this issue, we used a multiple threshold approach to identify species-specific profiles of proteome complexity and, by comparing properties of these sets, determine the influence that starting parameters have on evolutionary inferences.ResultsWe find that, although qualitatively similar, quantitatively each species has a unique LCR profile which represents the frequency of these regions within each genome. Inferences based on these profiles are more accurate in comparative analyses of genome complexity as they allow to determine the relative complexity of multiple genomes as well as the type of repetitiveness that is most common in each. Based on the multiple threshold LCR sets obtained, we identified predominant evolutionary mechanisms at different complexity levels, which show neutral mechanisms acting on highly repetitive LCRs (e.g., homopolymers) and selective forces becoming more important as heterogeneity of the LCRs increases.ConclusionsOur results show how inferences based on LCRs are influenced by the parameters used to identify these regions. Sets of LCRs are heterogeneous aggregates of regions that include homo- and heteropolymers and, as such, evolve according to different mechanisms. LCR profiles provide a new way to investigate genome complexity across species and to determine the driving mechanism of their evolution.

【 授权许可】

CC BY   
© Battistuzzi et al. 2016

【 图 表 】

Fig. 1