Survey of artemisinin production by diverse Artemisia species in northern Pakistan

Malaria Journal

Research

Muhammad F Asim¹  Bushra Mirza¹  Waheed Arshad¹  Abdul Mannan²  Ibrar Ahmed³  Izhar Hussain⁴  Rizwana A Qureshi⁵ 

[1] Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan;Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan;Department of Pharmaceutical Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan;Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan;Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand;Department of Pharmaceutical Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan;Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan;

关键词: High Performance Liquid Chromatography;    Malaria;    Glandular Trichome;    Artemisinin Combination Therapy;    Artemisinin Content;   

DOI  :  10.1186/1475-2875-9-310

 received in 2010-09-23, accepted in 2010-11-04,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundArtemisinin is the current drug of choice for treatment of malaria and a number of other diseases. It is obtained from the annual herb, Artemisia annua and some microbial sources by genetic engineering. There is a great concern that the artemisinin production at current rate will not meet the increasing demand by the pharmaceutical industry, so looking for additional sources is imperative.MethodsIn current study, artemisinin concentration was analysed and compared in the flowers, leaves, roots and stems of Artemisia annua and 14 other Artemisia species including two varieties each for Artemisia roxburghiana and Artemisia dracunculus using high performance liquid chromatography (HPLC).ResultsThe highest artemisinin concentration was detected in the leaves (0.44 ± 0.03%) and flowers (0.42 ± 0.03%) of A. annua, followed by the flowers (0.34 ± .02%) of A. bushriences and leaves (0.27 ± 0%) of A. dracunculus var dracunculus. The average concentration of artemisinin varied in the order of flowers > leaves > stems > roots.ConclusionThis study identifies twelve novel plant sources of artemisinin, which may be helpful for pharmaceutical production of artemisinin. This is the first report of quantitative comparison of artemisinin among a large number of Artemisia species.

【 授权许可】

CC BY   
© Mannan et al; licensee BioMed Central Ltd. 2010