Differential detection of alternatively spliced variants of Ciz1 in normal and cancer cells using a custom exon-junction microarray

BMC Cancer

Research Article

Naveed Aziz¹  Dawn Coverley¹  Faisal A Rahman² 

[1] Department of Biology, University of York, UK;Department of Biology, University of York, UK;Parkwood Hospital, Blackpool, UK;

关键词: Matrin;    Alternative Splice Event;    Alternative Exon;    EWSR1 Gene;    Junction Probe;   

DOI  :  10.1186/1471-2407-10-482

 received in 2009-08-28, accepted in 2010-09-10,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCiz1 promotes initiation of mammalian DNA replication and is present within nuclear matrix associated DNA replication factories. Depletion of Ciz1 from normal and cancer cells restrains entry to S phase and inhibits cell proliferation. Several alternative splicing events with putative functional consequences have been identified and reported, but many more variants are predicted to exist based on publicly available mRNAs and expressed sequence tags.MethodsHere we report the development and validation of a custom exon and exon-junction microarray focused on the human CIZ1 gene, capable of reproducible detection of differential splice-variant expression.ResultsUsing a pair of paediatric cancer cell lines and a pool of eight normal lines as reference, the array identified expected and novel CIZ1 splicing events. One novel variant (delta 8-12) that encodes a predicted protein lacking key functional sites, was validated by quantitative RT-PCR and found to be over-represented in a range of other cancer cell lines, and over half of a panel of primary lung tumours.ConclusionsExpression of CIZ1 delta 8-12 appears to be restricted to cancer cells, and may therefore be a useful novel biomarker

【 授权许可】

Unknown   
© Rahman et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.