Evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis

Journal of Cardiovascular Magnetic Resonance

Research

Slobodan I Macura¹  Bruce E Knudsen²  Joseph P Grande²  Lilach O Lerman³  Behzad Ebrahimi³  John A Crane³ 

[1] Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA;Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA;Division of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, USA;

关键词: Renal artery stenosis;    LV dysfunction;    Ultra-high field MRI;    BOLD;    ASL;    Hypoxia;   

DOI  :  10.1186/1532-429X-15-98

 received in 2012-12-21, accepted in 2013-10-16,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundRenal artery stenosis (RAS) promotes hypertension and cardiac dysfunction. The 2-kidney, 1-clip mouse model in many ways resembles RAS in humans and is amenable for genetic manipulation, but difficult to evaluate noninvasively. We hypothesized that cardiovascular magnetic resonance (CMR) is capable of detecting progressive cardiac and renal dysfunction in mice with RAS and monitoring the progression of the disease longitudinally.MethodsRAS was induced at baseline in eighteen mice by constricting the renal artery. Nine additional animals served as normal controls. CMR scans (16.4 T) were performed in all mice one week before and 2 and 4 weeks after baseline. Renal volumes and hemodynamics were assessed using 3D fast imaging with steady-state precession and arterial spin labelling, and cardiac function using CMR cine. Renal hypoxia was investigated using blood oxygen-level dependent (BOLD) MR.ResultsTwo weeks after surgery, mean arterial pressure was elevated in RAS mice. The stenotic kidney (STK) showed atrophy, while the contra-lateral kidney (CLK) showed hypertrophy. Renal blood flow (RBF) and cortical oxygenation level declined in the STK but remained unchanged in CLK. Moreover, cardiac end-diastolic and stroke volumes decreased and myocardial mass increased. At 4 weeks, STK RBF remained declined and the STK cortex and medulla showed development of hypoxia. Additionally, BOLD detected a mild hypoxia in CLK cortex. Cardiac end-diastolic and stroke volumes remained reduced and left ventricular hypertrophy worsened. Left ventricular filling velocities (E/A) indicated progression of cardiac dysfunction towards restrictive filling.ConclusionsCMR detected longitudinal progression of cardiac and renal dysfunction in 2K, 1C mice. These observations support the use of high-field CMR to obtain useful information regarding chronic cardiac and renal dysfunction in small animals.

【 授权许可】

Unknown   
© Ebrahimi et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.