The association of the Clock 3111 T/C SNP with lipids and lipoproteins including small dense low-density lipoprotein: results from the Mima study

BMC Medical Genetics

Research Article

Yoshiko Sano¹  Kaoru Takahashi¹  Kokoro Tsuzaki¹  Naoki Sakane¹  Kazuhiko Kotani¹  Shinji Fujiwara² 

[1] Division of Preventive Medicine and Diabetes Education, Clinical Research Institute for Endocrine and Metabolic Disease, National Hospital Organization Kyoto Medical Center, Kyoto, Japan;Mima City National Health Insurance Koyadaira Clinic, Tokushima, Japan;

关键词: Single Nucleotide Polymorphism;    Clock Gene;    Stepwise Multiple Regression Analysis;    Clock Protein;    Cardiometabolic Disorder;   

DOI  :  10.1186/1471-2350-11-150

 received in 2010-06-01, accepted in 2010-10-21,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe clock molecule plays major roles in circadian rhythmicity and regulating lipid and glucose metabolism in peripheral organs. Disruption of the circadian rhythm can lead to cardiometabolic disorders. The existence of small dense low-density lipoprotein (sdLDL) in the circulation, an abnormality of lipid metabolism, in part associated with lifestyle, is also one of risk parameters for cardiometabolic disorders. The 3111 T/C single nucleotide polymorphism (SNP) of the Clock gene has been reported to be associated with lifestyle including morning/evening preference. We investigated whether the Clock 3111 T/C SNP may affect lipids and lipoproteins including sdLDL.MethodsIn 365 community-dwelling subjects (170 men and 195 women, mean age 63 ± 14 years), the 3111 T/C SNP was genotyped using a fluorescent allele-specific DNA primer assay system. The levels of sdLDL were measured with the electrophoretic separation of lipoproteins employing the Lipoprint system.ResultsThe frequency of the Clock 3111 C allele was 0.14. The area of sdLDL did not differ between the subjects with obesity and those without. In carriers of T/T homozygotes, the area of sdLDL was significantly higher compared with carriers of the C allele (T/C or C/C) (1.7 ± 3.4 vs. 0.8 ± 1.9%; p < 0.05). A multiple regression analysis showed that the area of sdLDL was significantly and negatively correlated with the Clock 3111 T/C SNP (β = -0.114, p < 0.05), independently of age, sex, body mass index, and exercise habits.ConclusionOur findings indicated that the Clock 3111 T/C SNP might be associated with the existence of sdLDL.

【 授权许可】

Unknown   
© Tsuzaki et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.