Helicobacter pylori with stronger intensity of CagA phosphorylation lead to an increased risk of gastric intestinal metaplasia and cancer

BMC Microbiology

Research Article

Chiao-Hsiung Chuang¹  Bor-Shyang Sheu²  Wei-Lun Chang³  Hsiu-Chi Cheng³  Hsiao-Bai Yang⁴  Jiunn-Jong Wu⁵  Shew-Meei Sheu⁵  Kuei-Hsiang Hung⁵ 

[1]Department of Internal Medicine, Medical College, National Cheng Kung University, Sheng-Li Road, Tainan, Taiwan

[2]Department of Internal Medicine, Medical College, National Cheng Kung University, Sheng-Li Road, Tainan, Taiwan

[3]Institute of Basic Medical Sciences, Medical College, National Cheng Kung University, Sheng-Li Road, Tainan, Taiwan

[4]Institute of Clinical Medicine, Medical College, National Cheng Kung University, Sheng-Li Road, Tainan, Taiwan

[5]Department of Internal Medicine, Medical College, National Cheng Kung University, Sheng-Li Road, Tainan, Taiwan

[6]Institute of Clinical Medicine, Medical College, National Cheng Kung University, Sheng-Li Road, Tainan, Taiwan

[7]Department of Pathology, Medical College, National Cheng Kung University, Sheng-Li Road, Tainan, Taiwan

[8]Institute of Basic Medical Sciences, Medical College, National Cheng Kung University, Sheng-Li Road, Tainan, Taiwan

关键词: H. pylori;    cagA;    CagA phosphorylation;    intestinal metaplasia;    gastric cancer;   

DOI  :  10.1186/1471-2180-11-121

 received in 2010-11-27, accepted in 2011-05-27,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundNearly all Taiwanese H. pylori stains are cagA-genopositive and encode CagA protein. In this study, we evaluated whether different intensity of tyrosine phosphorylated-CagA (p-CagA) had an impact on the clinical diseases and histological outcomes in this area.ResultsWe enrolled 469 dyspeptic patients and prospectively obtained the gastric biopsy specimens and the H. pylori isolates. These patients were categorized according to the clinical diseases, such as duodenal ulcer, gastric ulcer, gastric cancer, and gastritis with or without intestinal metaplasia. Their gastric specimens were reviewed by the updated Sydney's system. Furthermore, a total of 146 patients were randomly selected from each clinical category for evaluation of their isolates' p-CagA intensity by in vitro AGS cells co-culture. The p-CagA was sparse in 30 (20.5%), weak in 59 (40.5%), and strong in 57 (39%) isolates. The isolates from the patients of gastric cancer or gastritis with intestinal metaplasia had stronger p-CagA intensity than those of gastritis without intestinal metaplasia (p ≤ 0.002). Moreover, the patients infected with isolates with strong or weak p-CagA intensity had a higher risk of gastric intestinal metaplasia (p < 0.05, odds ratio 3.09~15.26) than those infected with sparse p-CagA isolates.ConclusionsInfection with H. pylori stains with stronger p-CagA intensity may lead to an increased risk of gastric intestinal metaplasia and cancer.

【 授权许可】

Unknown   
© Chuang et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.