Impact of the LDL subfraction phenotype on Lp-PLA2 distribution, LDL modification and HDL composition in type 2 diabetes

Cardiovascular Diabetology

Original Investigation

Elena De Juan-Franco¹  Jose Luis Sánchez-Quesada¹  Rosa Bonet-Marques¹  Jordi Ordóñez-Llanos²  Juan Sánchez-Hernández³  Francisco Blanco-Vaca⁴  Irene Vinagre⁵  Antonio Pérez⁶ 

[1] Biomedical Research Institute IIB Sant Pau, Cardiovascular Biochemistry Group, C/ Antoni Maria Claret, 167, 08025, Barcelona, Spain;Biomedical Research Institute IIB Sant Pau, Cardiovascular Biochemistry Group, C/ Antoni Maria Claret, 167, 08025, Barcelona, Spain;Biochemistry and Molecular Biology Department, Universitat Autònoma de Barcelona, Cerdanyola del Vallés, Spain;CIBER for the Study of Diabetes and Associated Metabolic Diseases (CIBERDEM), Barcelona, Spain;CIBER for the Study of Diabetes and Associated Metabolic Diseases (CIBERDEM), Barcelona, Spain;Metabolic Factors of Cardiovascular Risk Group, Biomedical Research Institute IIB Sant Pau, Barcelona, Spain;Biochemistry and Molecular Biology Department, Universitat Autònoma de Barcelona, Cerdanyola del Vallés, Spain;Endocrinology and Nutrition Department, Hospital de la Santa Creu i Sant Pau, C/ Mas Casanovas, 90, 08041, Barcelona, Spain;Endocrinology and Nutrition Department, Hospital de la Santa Creu i Sant Pau, C/ Mas Casanovas, 90, 08041, Barcelona, Spain;CIBER for the Study of Diabetes and Associated Metabolic Diseases (CIBERDEM), Barcelona, Spain;

关键词: Low-density lipoprotein;    High-density lipoprotein;    Lipoprotein-associated phospholipase A2;    Modified LDL;    Type 2 diabetes;    LDL subfraction phenotype;   

DOI  :  10.1186/1475-2840-12-112

 received in 2013-06-06, accepted in 2013-08-03,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundQualitative alterations of lipoproteins underlie the high incidence of atherosclerosis in diabetes. The objective of this study was to assess the impact of low-density lipoprotein (LDL) subfraction phenotype on the qualitative characteristics of LDL and high-density lipoprotein (HDL) in patients with type 2 diabetes.MethodsOne hundred twenty two patients with type 2 diabetes in poor glycemic control and 54 healthy subjects were included in the study. Patients were classified according to their LDL subfraction phenotype. Seventy-seven patients presented phenotype A whereas 45 had phenotype B. All control subjects showed phenotype A. Several forms of modified LDL, HDL composition and the activity and distribution of lipoprotein-associated phospholipase A2 (Lp-PLA2) were analyzed.ResultsOxidized LDL, glycated LDL and electronegative LDL were increased in both groups of patients compared with the control group. Patients with phenotype B had increased oxidized LDL and glycated LDL concentration than patients with phenotype A. HDL composition was abnormal in patients with diabetes, being these abnormalities more marked in patients with phenotype B. Total Lp-PLA2 activity was higher in phenotype B than in phenotype A or in control subjects. The distribution of Lp-PLA2 between HDL and apoB-containing lipoproteins differed in patients with phenotype A and phenotype B, with higher activity associated to apoB-containing lipoproteins in the latter.ConclusionsThe presence of LDL subfraction phenotype B is associated with increased oxidized LDL, glycated LDL and Lp-PLA2 activity associated to apoB-containing lipoproteins, as well as with abnormal HDL composition.

【 授权许可】

CC BY   
© Sánchez-Quesada et al.; licensee BioMed Central Ltd. 2013