Comparative susceptibility to Plasmodium falciparum of the molecular forms M and S of Anopheles gambiae and Anopheles arabiensis

Malaria Journal

Research

Lassana Konate¹  Ousmane Faye¹  Anna Cohuet²  Cheikh Sokhna³  Catherine Mazenot³  Mamadou O Ndiath³  Jean-François Trape³  Christian Boudin³  Ablaye Gaye⁴ 

[1] Laboratoire Ecologie Vectorielle et Parasitaire, UCAD, BP 5005, Fann Dakar, Sénégal;Maladies infectieuses et vecteurs: écologie, génétique, évolution et contrôle (MIVEGEC), (UM1-CNRS 5290-IRD 224), IRD-IRSS, 01 BP 171, Bobo Dioulasso, Burkina Faso;Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), IRD, BP 1386, Dakar, Sénégal;Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), IRD, BP 1386, Dakar, Sénégal;Laboratoire Ecologie Vectorielle et Parasitaire, UCAD, BP 5005, Fann Dakar, Sénégal;

关键词: Malaria;    Malaria Transmission;    Molecular Form;    Plasmodium Infection;    Anopheles Arabiensis;   

DOI  :  10.1186/1475-2875-10-269

 received in 2011-06-28, accepted in 2011-09-19,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe different taxa belonging to Anopheles gambiae complex display phenotypic differences that may impact their contribution to malaria transmission. More specifically, their susceptibility to infection, resulting from a co-evolution between parasite and vector, might be different. The aim of this study was to compare the susceptibility of M and S molecular forms of Anopheles gambiae and Anopheles arabiensis to infection by Plasmodium falciparum.MethodsF3 progenies of Anopheles gambiae s.l. collected in Senegal were infected, using direct membrane feeding, with P. falciparum gametocyte-containing blood sampled on volunteer patients. The presence of oocysts was determined by light microscopy after 7 days, and the presence of sporozoite by ELISA after 14 days. Mosquito species and molecular forms were identified by PCR.ResultsThe oocyst rate was significantly higher in the molecular S form (79.07%) than in the M form (57.81%, Fisher's exact test p < 0.001) and in Anopheles arabiensis (55.38%, Fisher's exact test vs. S group p < 0.001). Mean ± s.e.m. number of oocyst was greater in the An. gambiae S form (1.72 ± 0.26) than in the An. gambiae M form (0.64 ± 0.04, p < 0.0001) and in the An. arabiensis group (0.58 ± 0.04, vs. S group, p < 0.0001). Sporozoite rate was also higher in the molecular form S (83.52%) than in form M (50.98%, Fisher's exact test p < 0.001) and Anopheles arabiensis 50.85%, Fisher's exact test vs. S group p < 0.001).ConclusionInfected in the same experimental conditions, the molecular form S of An. gambiae is more susceptible to infection by P. falciparum than the molecular form M of An. gambiae and An. arabiensis.

【 授权许可】

CC BY   
© Ndiath et al; licensee BioMed Central Ltd. 2011