The effect of nanoparticle size on the probability to cross the blood-brain barrier: an in-vitro endothelial cell model

Journal of Nanobiotechnology

Research

Rachela Popovtzer¹  Malka Shilo¹  Koby Baranes¹  Menachem Motiei¹  Anat Sharon²  Jean-Paul M Lellouche² 

[1] Faculty of Engineering & the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, 52900, Ramat Gan, Israel;The Department of Chemistry & the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, 52900, Ramat Gan, Israel;

关键词: Bloodbrain Barrier;    Gold Nanoparticles;    Barbiturate;    bEnd.3 cells;    Nanoparticle size;   

DOI  :  10.1186/s12951-015-0075-7

 received in 2014-11-21, accepted in 2015-01-31,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundDuring the last decade nanoparticles have gained attention as promising drug delivery agents that can transport through the blood brain barrier. Recently, several studies have demonstrated that specifically targeted nanoparticles which carry a large payload of therapeutic agents can effectively enhance therapeutic agent delivery to the brain. However, it is difficult to draw definite design principles across these studies, owing to the differences in material, size, shape and targeting agents of the nanoparticles. Therefore, the main objective of this study is to develop general design principles that link the size of the nanoparticle with the probability to cross the blood brain barrier. Specifically, we investigate the effect of the nanoparticle size on the probability of barbiturate coated GNPs to cross the blood brain barrier by using bEnd.3 brain endothelial cells as an in vitro blood brain barrier model.ResultsThe results show that GNPs of size 70 nm are optimal for the maximum amount of gold within the brain cells, and that 20 nm GNPs are the optimal size for maximum free surface area.ConclusionsThese findings can help understand the effect of particle size on the ability to cross the blood brain barrier through the endothelial cell model, and design nanoparticles for brain imaging/therapy contrast agents.

【 授权许可】

Unknown   
© Shilo et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.