Immature rat seminal vesicles show histomorphological and ultrastructural alterations following treatment with kisspeptin-10

Reproductive Biology and Endocrinology

Research

Muhammad Ramzan¹  Faiqah Ramzan²  Muhammad Haris Ramzan³  Irfan Zia Qureshi⁴  Faiza Ramzan⁵ 

[1] Department of Biochemistry, Peshawar Medical College, Peshawar, Pakistan;Gomal Centre of Biochemistry and Biotechnology, Gomal University, 29050, Dera Ismail Khan, Pakistan;Laboratory of Animal Physiology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan;Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan;Laboratory of Animal Physiology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan;Laboratory of Microbiology, Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan;

关键词: Seminal Vesicle;    Secretory Granule;    Principal Cell;    Central Precocious Puberty;    Epithelial Height;   

DOI  :  10.1186/1477-7827-10-18

 received in 2011-10-12, accepted in 2012-03-10,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundDegenerative effects of critical regulators of reproduction, the kisspeptin peptides, on cellular aspects of sexually immature male gonads are known but similar information on accessory sex glands remain elusive.MethodsPrepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day. Control rats were maintained in parallel. The day following the end of the experimental period, seminal vesicles were removed and processed for light and electron microscopic examination using the standard methods. DNA damage was estimated by DNA ladder assay and DNA fragmentation assay.ResultsThe results demonstrated cellular degeneration. Epithelial cell height of seminal vesicles decreased significantly at all doses (P < 0.05). Marked decrease in epithelial folds was readily noticeable, while the lumen was dilated. Ultrastructural changes were characterized by dilatation of endoplasmic reticulum and Golgi complex, heterochromatization of nuclei, invagination of nuclear membranes and a decreased number of secretory granules. Percent DNA damage to the seminal vesicle was 19.54 +/- 1.98, 38.06 +/- 2.09 and 58.18 +/- 2.59 at 10 pg, 1 ng and 1 microgram doses respectively.ConclusionThe study reveals that continuous administration of kisspeptin does not lead to an early maturation but instead severe degeneration of sexually immature seminal vesicles.

【 授权许可】

CC BY   
© Ramzan et al; licensee BioMed Central Ltd. 2012