The role of GAGE cancer/testis antigen in metastasis: the jury is still out

BMC Cancer

Research Article

Morten Frier Gjerstorff¹  Mikkel Green Terp¹  Malene Bredahl Hansen¹  Henrik Jørn Ditzel² 

[1] Department of Cancer and Inflammation Research, Institute for Molecular Medicine, University of Southern Denmark, Winsloewparken 25, 3, DK-5000, Odense, Denmark;Department of Cancer and Inflammation Research, Institute for Molecular Medicine, University of Southern Denmark, Winsloewparken 25, 3, DK-5000, Odense, Denmark;Department of Oncology, Odense University Hospital, Sdr. Boulevard 29, DK-5000, Odense, Denmark;

关键词: Immunodeficient Mouse;    Gage Protein;    CL16 Cell;    shRNA Plasmid;    Gage Expression;   

DOI  :  10.1186/s12885-015-1998-y

 received in 2015-03-25, accepted in 2015-12-11,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundGAGE cancer/testis antigens are frequently expressed in various types of malignancies and represent attractive targets for immunotherapy, however their role in cancer initiation and progression has remained elusive. GAGE proteins are expressed in normal cells during early development with migratory and invasive properties and were found to be upregulated in cancer cells with metastasizing potential in a gastric cancer model.MethodsWe have addressed the direct role of GAGE proteins in supporting metastasis using an isogenic metastasis model of human cancer, consisting of 4 isogenic cell lines, which are equally tumorigenic in immunodeficient mice, but differ with their ability to generate metastases in the lungs and lymph nodes.ResultsAlthough GAGE proteins were strongly upregulated in the highly metastatic clone (CL16) compared to non-metastatic (NM2C5), weakly metastatic (M4A4) and moderately metastatic clones (LM3), stable downregulation of GAGE expression did not affect the ability of CL16 cells to establish primary tumors and form metastasis in the lungs of immunodeficient mice.ConclusionsThese results suggest that GAGE proteins per se do not support metastasis and that further studies are needed to clarify the contribution of GAGE proteins to the metastatic potential of different types of cancer cells.

【 授权许可】

CC BY   
© Gjerstorff et al. 2016