Prevalence and genetic characterization of Cryptosporidium species and Giardia duodenalis in lambs in Oromia Special Zone, Central Ethiopia

BMC Veterinary Research

Research Article

Berhanu Erko¹  Getachew Tilahun¹  Teklu Wegayehu²  Junqiang Li³  Longxian Zhang³  Md Robiul Karim⁴  Haileeyesus Adamu⁵ 

[1] Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia;Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia;College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia;College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China;College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China;Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, 1706, Gazipur, Bangladesh;Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia;

关键词: Cryptosporidium;    Giardia duodenalis;    Zoonotic transmission;    Lamb;    Ethiopia;   

DOI  :  10.1186/s12917-016-0916-0

 received in 2016-01-23, accepted in 2016-12-06,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCryptosporidium and Giardia duodenalis are gastro-intestinal parasites that infect human and animals worldwide. Both parasites share a broad host range and are believed to be zoonosis. The aim of this study was to identify the species of Cryptosporidium and assemblages of G. duodenalis in lambs and to elucidate their role in zoonotic transmission.ResultsA total of 389 fecal samples were collected from lambs and screened by microscopy and nested PCR targeting the small-subunit ribosomal RNA for Cryptosporidium; and the small-subunit ribosomal RNA, triose phosphate isomerase, β-giardin, and glutamate dehydrogenase genes for G. duodenalis. The prevalence of Cryptosporidium and G. duodenalis was 2.1% (8/389) and 2.6% (10/389), respectively. The infection rate at the three study sites ranged from 1.3 to 3.1% for Cryptosporidium and 1.6 to 3.9% for G. duodenalis; but variation was not statistically significant (p > 0.05). The finding also showed that there is no sex and age group associated difference in the occurrence of Cryptosporidium and G. duodenalis infections in lambs. Sequence analysis revealed that lambs were mono-infection with C. ubiquitum and G. duodenalis assemblage E. The analysis also indicated the presence of genetic variation within isolates of assemblage E; with 4 of them are novel genotypes at the small-subunit ribosomal RNA, β-giardin, and glutamate dehydrogenase genes.ConclusionThe findings of the current study showed that lambs are capable of harboring C. ubiquitum and G. duodenalis assemblage E. This finding suggests that lambs might be sources for potentially zoonotic Cryptosporidium species. This was first molecular study in lambs and contributes to a better understanding of the epidemiology of Cryptosporidium and G. duodenalis in central Ethiopia.

【 授权许可】

CC BY   
© The Author(s). 2017