Efficient digest of high-throughput sequencing data in a reproducible report

BMC Bioinformatics

Research

Zhe Zhang¹  Ariella Sasson¹  Juan C Perin¹  Mahdi Sarmady¹  Angela M Yu¹  Patrick V Warren¹  Hongbo M Xie¹  Jeremy Leipzig¹  Peter S White² 

[1] Center for Biomedical Informatics, The Children's Hospital of Philadelphia, PA, USA;Center for Biomedical Informatics, The Children's Hospital of Philadelphia, PA, USA;Division of Oncology, The Children's Hospital of Philadelphia, PA, USA;Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, PA, USA;

关键词: Read Count;    Sequencing Depth;    Sequencing Quality;    Alignment Program;    Bioconductor Project;   

DOI  :  10.1186/1471-2105-14-S11-S3

来源: Springer

【 摘 要 】

BackgroundHigh-throughput sequencing (HTS) technologies are spearheading the accelerated development of biomedical research. Processing and summarizing the large amount of data generated by HTS presents a non-trivial challenge to bioinformatics. A commonly adopted standard is to store sequencing reads aligned to a reference genome in SAM (Sequence Alignment/Map) or BAM (Binary Alignment/Map) files. Quality control of SAM/BAM files is a critical checkpoint before downstream analysis. The goal of the current project is to facilitate and standardize this process.ResultsWe developed bamchop, a robust program to efficiently summarize key statistical metrics of HTS data stored in BAM files, and to visually present the results in a formatted report. The report documents information about various aspects of HTS data, such as sequencing quality, mapping to a reference genome, sequencing coverage, and base frequency. Bamchop uses the R language and Bioconductor packages to calculate statistical matrices and the Sweave utility and associated LaTeX markup for documentation. Bamchop's efficiency and robustness were tested on BAM files generated by local sequencing facilities and the 1000 Genomes Project. Source code, instruction and example reports of bamchop are freely available from https://github.com/CBMi-BiG/bamchop.ConclusionsBamchop enables biomedical researchers to quickly and rigorously evaluate HTS data by providing a convenient synopsis and user-friendly reports.

【 授权许可】

CC BY   
© Zhang et al; licensee BioMed Central Ltd. 2013