Identification of the estrogen receptor GPER in neoplastic and non-neoplastic human testes

Reproductive Biology and Endocrinology

Research

Francesca Giordano¹  Vittoria Rago¹  Amalia Carpino¹  Marcello Maggiolini²  Francesco Romeo³ 

[1] Department of Cell Biology, Faculty of Pharmacy, University of Calabria, Italy;Department of Pharmaco-Biology, Faculty of Pharmacy, University of Calabria, Italy;Pathologic Anatomy Unit, Annunziata Hospital, Cosenza, Italy;

关键词: Estrogen Receptor;    Germ Cell;    Sertoli Cell;    Leydig Cell;    Seminiferous Tubule;   

DOI  :  10.1186/1477-7827-9-135

 received in 2011-06-27, accepted in 2011-10-05,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundEstrogen signaling is mediated by estrogen receptor beta isoforms in normal and neoplastic human testes. Recently, a G-protein-coupled-receptor (GPER) has been suggested as being involved in rapid responses to estrogens in different normal and tumor cells.MethodsThis study investigated the GPER expression in paraffin-embedded samples from non neoplastic and neoplastic human testes (sex-cord stromal and germ cell tumors) by immunohistochemical and Western Blot analyses.ResultsIn control testes, a positive GPER immunoreactivity was detected in Leydig and in Sertoli cells while all germ cells were immunonegative. Furthermore, neoplastic cells of the Sertoli cell tumor, Leydig cell tumor, seminoma and embryonal carcinoma samples were all immunopositive. The immunoblots of testis extracts confirmed the results.ConclusionsThese findings suggest that GPER could mediate estrogen signaling in both normal and transformed somatic cells of human testis, but they reveal a differential expression of the novel estrogen receptor in non neoplastic and neoplastic germ cells.

【 授权许可】

Unknown   
© Rago et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.