Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium

Journal of Biomedical Science

Research

Xiaofeng Ren¹  Yan Zhang²  Bin Zeng²  Guosheng Lin²  Honglei Chen³ 

[1] College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China;Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China;Department of Pathology, School of Basic Medical Science, Wuhan University, Wuhan, Hubei, China;

关键词: Vascular Endothelial Growth Factor;    Capillary Density;    Recombinant Adenovirus;    Angiogenic Growth Factor;    Angiogenic Effect;   

DOI  :  10.1186/1423-0127-17-80

 received in 2010-06-21, accepted in 2010-10-07,  发布年份 2010

来源: Springer

【 摘 要 】

Heme oxygenase-1 (HO-1) is a stress-inducible enzyme with diverse cytoprotective effects, and reported to have an important role in angiogenesis recently. Here we investigated whether HO-1 transduced by mesenchymal stem cells (MSCs) can induce angiogenic effects in infarcted myocardium. HO-1 was transfected into cultured MSCs using an adenoviral vector. 1 × 106 Ad-HO-1-transfected MSCs (HO-1-MSCs) or Ad-Null-transfected MSCs (Null-MSCs) or PBS was respectively injected into rat hearts intramyocardially at 1 h post-myocardial infarction. The results showed that HO-1-MSCs were able to induce stable expression of HO-1 in vitro and in vivo. The capillary density and expression of angiogenic growth factors, VEGF and FGF2 were significantly enhanced in HO-1-MSCs-treated hearts compared with Null-MSCs-treated and PBS-treated hearts. However, the angiogenic effects of HO-1 were abolished by treating the animals with HO inhibitor, zinc protoporphyrin. The myocardial apoptosis was marked reduced with significantly reduced fibrotic area in HO-1-MSCs-treated hearts; Furthermore, the cardiac function and remodeling were also significantly improved in HO-1-MSCs-treated hearts. Our current findings support the premise that HO-1 transduced by MSCs can induce angiogenic effects and improve heart function after acute myocardial infarction.

【 授权许可】

CC BY   
© Zeng et al; licensee BioMed Central Ltd. 2010