Dietary supplementation with arachidonic acid increases arachidonic acid content in paw, but does not affect arthritis severity or prostaglandin E2 content in rat adjuvant-induced arthritis model

Lipids in Health and Disease

Research

Ikuo Morita¹  Saki Kakutani²  Hiroshi Shibata²  Yoshihisa Kaneda²  Hiroshi Kawashima²  Norifumi Tateishi³ 

[1] Department of Cellular Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan;Institute for Health Care Science, Suntory Wellness Ltd., 1-1-1 Wakayamadai, 6188503, Shimamoto, Osaka, Japan;Institute for Health Care Science, Suntory Wellness Ltd., 1-1-1 Wakayamadai, 6188503, Shimamoto, Osaka, Japan;Department of physiology and pharmacology, School of advanced science and engineering, Waseda University, Tokyo, Japan;

关键词: Arachidonic acid;    Arthritis;    Prostaglandin E;    Lipoxin A;   

DOI  :  10.1186/1476-511X-14-3

 received in 2014-10-17, accepted in 2015-01-07,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundArachidonic acid (ARA) is an essential fatty acid and a major constituent of biomembranes. It is converted into various lipid mediators, such as prostaglandin E2 (PGE2), which is involved in the development of rheumatoid arthritis (RA). However, the effects of dietary ARA on RA are unclear. Our objective was to clarify the effects of dietary ARA on an experimental rat arthritis model.MethodsLew rats were fed three contents of ARA diet (0.07%, 0.15% or 0.32% ARA in diet (w/w)), a docosahexaenoic acid (DHA) diet (0.32% DHA), or a control diet. After 4 weeks, arthritis was induced by injection of Freund’s complete adjuvant into the hind footpad. We observed the development of arthritis for another 4 weeks, and evaluated arthritis severity, fatty acid and lipid mediator contents in the paw, and expression of genes related to lipid mediator formation and inflammatory cytokines. Treatment with indomethacin was also evaluated.ResultsThe ARA content of phospholipids in the paw was significantly elevated with dietary ARA in a dose-dependent manner. Dietary ARA as well as DHA did not affect arthritis severity (paw edema, arthritis score, and bone erosion). PGE2 content in the paw was increased by arthritis induction, but was not modified by dietary ARA. Dietary ARA did not affect the contents of other lipid mediators and gene expression of cyclooxygenase (COX)-1, COX-2, lipoxgenases and inflammatory cytokines. Indomethacin suppressed arthritis severity and PGE2 content in the paw.ConclusionThese results suggest that dietary ARA increases ARA content in the paw, but has no effect on arthritis severity and PGE2 content of the paw in a rat arthritis model.

【 授权许可】

CC BY   
© Tateishi et al.; licensee BioMed Central. 2015