Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration

BMC Biology

Research Article

Stefan Wiemann¹  Stephanie Bechtel¹  Victor Racine²  Jennifer L Rohn³  Buzz Baum³  Maria Teresa Herrera-Abreu⁴  Anne J Ridley⁴  Narendra Suryavanshi⁴  Virginia Tajadura⁴  Siau Wei Bai⁵ 

[1] Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, Heidelberg, Germany;Institute of Molecular and Cellular Biology, 61 Biopolis Drive, 138673, Proteos, Singapore;SWB, Institut Pasteur, 25-28 rue du Docteur Roux, 75015 PARIS; VR, Fluofarma, 2 rue Robert Escarpit, 33600, Pessac, France;MRC Laboratory of Molecular Cell Biology, University College London, Gower Street, WC1E 6BT, London, UK;Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, SE1 1UL, London, UK;Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, SE1 1UL, London, UK;SWB, Institut Pasteur, 25-28 rue du Docteur Roux, 75015 PARIS; VR, Fluofarma, 2 rue Robert Escarpit, 33600, Pessac, France;

关键词: Cell Migration;    HeLa Cell;    Down Syndrome;    Actin Filament;    Stress Fibre;   

DOI  :  10.1186/1741-7007-9-54

 received in 2011-03-10, accepted in 2011-08-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCell migration is essential during development and in human disease progression including cancer. Most cell migration studies concentrate on known or predicted components of migration pathways.ResultsHere we use data from a genome-wide RNAi morphology screen in Drosophila melanogaster cells together with bioinformatics to identify 26 new regulators of morphology and cytoskeletal organization in human cells. These include genes previously implicated in a wide range of functions, from mental retardation, Down syndrome and Huntington's disease to RNA and DNA-binding genes. We classify these genes into seven groups according to phenotype and identify those that affect cell migration. We further characterize a subset of seven genes, FAM40A, FAM40B, ARC, FMNL3, FNBP3/FBP11, LIMD1 and ZRANB1, each of which has a different effect on cell shape, actin filament distribution and cell migration. Interestingly, in several instances closely related isoforms with a single Drosophila homologue have distinct phenotypes. For example, FAM40B depletion induces cell elongation and tail retraction defects, whereas FAM40A depletion reduces cell spreading.ConclusionsOur results identify multiple regulators of cell migration and cytoskeletal signalling that are highly conserved between Drosophila and humans, and show that closely related paralogues can have very different functions in these processes.

【 授权许可】

Unknown   
© Bai et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.