Myocardial T1 maps reflect histological findings in acute and chronic stages of myocarditis in a rat model

Journal of Cardiovascular Magnetic Resonance

Research

Katharina Wassilew¹  Darach O h-Ici²  Tiago Ferreira da Silva²  Frédéric Münch²  Titus Kuehne²  Felix Berger²  Sarah Jeuthe³  Burkert Pieske³  Daniel R. Messroghli³ 

[1] Cardiovascular Pathology, Cardiothoracic and Vascular Surgery, Deutsches Herzzentrum Berlin, Berlin, Germany;Congenital Heart Disease and Pediatric Cardiology, Deutsches Herzzentrum Berlin, Berlin, Germany;Internal Medicine/Cardiology, Deutsches Herzzentrum Berlin, Augustenburger Platz 1, 13353, Berlin, Germany;

关键词: T1 mapping;    Autoimmune myocarditis;    Lewis rat;    Dilated cardiomyopathy;    Cardiovascular magnetic resonance;   

DOI  :  10.1186/s12968-016-0241-6

 received in 2016-01-15, accepted in 2016-03-31,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCardiovascular magnetic resonance offers both diagnostic and prognostic information in myocarditis. Using an established animal model of myocarditis, the aim of this study was to measure myocardial T1 before the onset, in the acute and in the chronic phases of the disease and to compare its course with histological and immunohistochemistry findings.MethodsMale young Lewis rats were immunized with 0.25 mg porcine myocardial myosin into the rear footpads on day 0. Native and contrast-enhanced ECG-triggered cardiac MRI examinations were performed before immunization on day 0 and on days 14, 21 and 35. Left ventricular function, pre- and post- contrast T1 parameters and LGE images were assessed using Small animal look-locker inversion recovery (SALLI). For each of the indicated time points a minimum of 4 rats were randomly sacrificed for pathological investigations including conventional histology (HE and Sirius-Red staining) and immunohistochemistry (CD 68) investigations.ResultsAll immunized rats developed myocarditis (morbidity 100 %). Histologically we observed increased wall thickness with biventricular macrophage-rich mixed inflammatory infiltrates. All rats with a histologically severe myocarditis showed increased native T1 and decreased post-contrast T1 of the myocardium.ConclusionsThe assessment of native T1 and post-contrast T1 allows accurate differentiation between healthy myocardium and myocardium with inflammation and also between the acute and chronic phases of the disease.

【 授权许可】

CC BY   
© Jeuthe et al. 2016