Fluorescent carbon dots as an efficient siRNA nanocarrier for its interference therapy in gastric cancer cells

Journal of Nanobiotechnology

Research

Chunlei Zhang¹  Hualin Fu¹  Huiyang Liu¹  Guangxia Shen¹  Daxiang Cui²  Qing Wang² 

[1] Department of Instrument Science & Engineering, Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, , School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan RD, 200240, Shanghai, China;School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China;Department of Instrument Science & Engineering, Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, , School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan RD, 200240, Shanghai, China;

关键词: Carbon dots;    siRNA interference therapy;    Gastric cancer;    Nanocarriers;   

DOI  :  10.1186/s12951-014-0058-0

 received in 2014-10-28, accepted in 2014-12-05,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundFluorescent carbon dots (Cdots) have attracted increasing attention due to their potential applications in sensing, catalysis, and biomedicine. Currently, intensive research has been concentrated on the synthesis and imaging-guided therapy of these benign photoluminescent materials. Meanwhile, Cdots have been explored as nonviral vector for nucleic acid or drug delivery by chemical modification on purpose.ResultsWe have developed a microwave assisted one-step synthesis of Cdots with citric acid as carbon source and tryptophan (Trp) as both nitrogen source and passivation agent. The Cdots with uniform size show superior water solubility, excellent biocompatibility, and high quantum yield. Afterwards, the PEI (polyethylenimine)-adsorbed Cdots nanoparticles (Cdots@PEI) were applied to deliver Survivin siRNA into human gastric cancer cell line MGC-803. The results have confirmed the nanocarrier exhibited excellent biocompatibility and a significant increase in cellular delivery of siRNA, inducing efficient knockdown for Survivin protein to 6.1%. In addition, PEI@Cdots complexes mediated Survivin silencing, the arrested cell cycle progression in G1 phase as well as cell apoptosis was observed.ConclusionThe Cdots-based and PEI-adsorbed complexes both as imaging agents and siRNA nanocarriers have been developed for Survivin siRNA delivery. And the results indicate that Cdots-based nanocarriers could be utilized in a broad range of siRNA delivery systems for cancer therapy.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.