Evaluation of antiaggregatory activity of flavonoid aglycone series

Nutrition Journal

Research

Siniša Tomić¹  Maja Tomičić²  Mirza Bojić³  Marica Medić-Šarić³  Željko Debeljak⁴ 

[1] Agency for Medicinal Products and Medical Devices of Croatia, Ksaverska cesta 4, Zagreb, Croatia;Croatian Institute of Transfusion Medicine, Department of Platelet and Leukocyte Immunology, Petrova 3, 10000, Zagreb, Croatia;Faculty of Pharmacy and Biochemistry, Department of Medicinal Chemistry, University of Zagreb, A. Kovačića 1, 10000, Zagreb, Croatia;Faculty of Pharmacy and Biochemistry, Department of Medicinal Chemistry, University of Zagreb, A. Kovačića 1, 10000, Zagreb, Croatia;Department of Clinical Laboratory Diagnostics, CHC Osijek, J. Huttlera 4, 31000, Osijek, Croatia;

关键词: Flavonoid;    Quercetin;    Clopidogrel;    Flavone;    Luteolin;   

DOI  :  10.1186/1475-2891-10-73

 received in 2011-03-15, accepted in 2011-07-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundAmong natural compounds, present in every day diet, flavonoids have shown beneficial effect in prevention of cardiovascular diseases that can be attributed, at least partially to the described antiaggregatory activity i.e. antiplatelet effects of flavonoids. Due to the ever increasing pharmacological interest in antiplatelet agents a systematic experimental evaluation of large flavonoid series is needed.MethodsA set of thirty flavonoid aglycones has been selected for the evaluation. All measurements of aggregation were done under standardized and firmly controlled in vitro conditions. The whole blood samples, multiple platelet functional analyzer and adenosine diphosphate (ADP) as a weak agonist of aggregation were selected for this purpose.ResultsThe results were expressed as minimal concentration of flavonoid that can significantly lower the platelet aggregation compared to the corresponding untreated sample (minimal antiaggregatory concentration - MINaAC). All analyzed flavonoids exhibited antiaggregatory activity MINaAC ranging from 0.119 μM to 122 μM, while the most potent representatives were 3,6-dihydroxyflavone (0.119 μM) and syringetin (0.119 μM).ConclusionsMeasurable antiplatelet activity established at submicromolar flavonoid concentrations suggests that even a dietary consumption of some flavonoids can make an impact on in vivo aggregation of platelets. These findings also point out a therapeutical potential of some flavonoids.

【 授权许可】

Unknown   
© Bojićć et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.