Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls

Proteome Science

Research

Chun-Feng Li¹  Jia-Ren Liu²  Shu-Jun Zhang³  Lei Wang³  Chun-Peng Zhang³  Jin-Lu Zhao³  Jun-Chao Jin³  Yan Liu³  Hong-Sheng Chen³  Ming Liu³  Luo-Qiang Lin³ 

[1] Department of Abdominal Surgery, the Affiliated Tumor Hospital of Harbin Medical University, 150081, Harbin, PR China;Public Health College, Harbin Medical University, 150081, Harbin, PR China;Harvard Medical School, 300 Longwood Ave, Boston, MA, USA;Treatment Center of Oncology, the Fourth Affiliated Hospital of Harbin Medical University, 150001, Harbin, PR China;

关键词: Colorectal Cancer;    Fecal Occult Blood Test;    Diagnostic Model;    Protein Chip;    Serum Proteome;   

DOI  :  10.1186/1477-5956-8-16

 received in 2010-01-05, accepted in 2010-03-25,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundColorectal cancer (CRC) is often diagnosed at a late stage with concomitant poor prognosis. The hypersensitive analytical technique of proteomics can detect molecular changes before the tumor is palpable. The surface-enhanced laser desorption/ionization-time of flight-mass spectra (SELDI-TOF-MS) is a newly-developed technique of evaluating protein separation in recent years. The protein chips have established the expression of tumor protein in the serum specimens and become the newly discovered markers for tumor diagnosis. The objective of this study was to find new markers of the diagnosis among groups of CRC, colorectal benign diseases (CBD) and healthy controls. The assay of SELDI-TOF-MS with analytical technique of protein-chip bioinformatics was used to detect the expression of protein mass peaks in the sera of patients or controls. One hundred serum samples, including 52 cases of colorectal cancer, 27 cases of colorectal benign disease, and 21 cases of healthy controls, were examined by SELDI-TOF-MS with WCX2 protein-chips.ResultsThe diagnostic models (I, II and III) were setup by analyzed the data and sieved markers using Ciphergen - Protein-Chip-Software 5.1. These models were combined with 3 protein mass peaks to discriminate CRC, CBD, and healthy controls. The accuracy, the sensitivity and the particularity of cross verification of these models are all highly over 80%.ConclusionsThe SELDI-TOF-MS is a useful tool to help diagnose colorectal cancer, especially during the early stage. However, identification of the significantly differentiated proteins needs further study.

【 授权许可】

CC BY   
© Liu et al; licensee BioMed Central Ltd. 2010