Efficient biodegradation of chlorophenols in aqueous phase by magnetically immobilized aniline-degrading Rhodococcus rhodochrous strain

Journal of Nanobiotechnology

Research

Bo Yu¹  Jianfeng Hou¹  Nan Wu²  Feixia Liu³  Jiansong Ju⁴ 

[1] CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, 100101, Chaoyang, Beijing, People’s Republic of China;CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, 100101, Chaoyang, Beijing, People’s Republic of China;College of Life Science, Hebei Normal University, 050024, Yuhua, Shijiazhuang, People’s Republic of China;CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, 100101, Chaoyang, Beijing, People’s Republic of China;University of Chinese Academy of Sciences, 100049, Shijingshan, Beijing, People’s Republic of China;College of Life Science, Hebei Normal University, 050024, Yuhua, Shijiazhuang, People’s Republic of China;

关键词: Chlorophenols;    Rhodococcus rhodochrous;    Magnetic immobilization;    Bioremediation;   

DOI  :  10.1186/s12951-016-0158-0

 received in 2015-10-16, accepted in 2016-01-10,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundChlorophenols are environmental contaminants, which are highly toxic to living beings due to their carcinogenic, mutagenic and cytotoxic properties. Bacterial degradation has been considered a cost-effective and eco-friendly method of removing chlorophenols, compared to the traditional physical–chemical processes.ResultsIn this study, we first developed an efficient process for the biodegradation of chlorophenols by magnetically immobilized Rhodococcus rhodochrous cells. R. rhodochrous DSM6263 degrades chlorophenols following the first step of hydroxylation at the ortho-positions of chlorophenolic rings. The cells immobilized by k-carrageenan with 9 g/L Fe3O4 nanoparticles could efficiently degrade 2-chlorophenol, 4-chlorophenol, 2,3-dichlorophenol and their mixture, which were even higher than those by free cells. The magnetically nanoparticle-immobilized cells could be used at least for six cycles.ConclusionGiven the much easier separation by an external magnetic field and high degradation efficiency, this study provides a promising technique for improving biocatalysts used in the bioremediation process for chlorophenols in wastewater.

【 授权许可】

CC BY   
© Hou et al. 2016