Origin recognition complex subunit 1(ORC1) is a potential biomarker and therapeutic target in cancer

BMC Medical Genomics

Research

Linling Wu¹  Hui Chen²  Chao Yang² 

[1] Integrated Chinese & Western Medicine Oncology Research Center, Jiangxi University of Traditional Chinese Medicine, 330004, Nanchang, China;Integrated Chinese & Western Medicine Oncology Research Center, Jiangxi University of Traditional Chinese Medicine, 330004, Nanchang, China;College of life science, Gannan Normal University, 341000, Ganzhou, China;

关键词: ORC1;    Pan-cancer;    Prognosis;    Immune cells;    Co-expression network;   

DOI  :  10.1186/s12920-023-01691-9

 received in 2023-01-30, accepted in 2023-10-05,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe origin recognition complex 1 (ORC1) is a large subunit of the origin recognition complex and acts as the master subunit of the precoding complex.ObjectiveTo explore potential function and clinical significance of ORC1 in cancers.MethodsThe expression level of ORC1 in different types of tumor tissues and matched normal tissues were detected by The Cancer Genome Atlas (TCGA) and validated by datasets from the gene expression omnibus (GEO) database. The association between ORC1 expression and infiltration levels of immune cell was analyzed. ORC1 and its co-expression genes were subjected to enrichment analysis to explore potential mechanisms in cancers, and the protein-protein interaction (PPI) network was constructed. Finally, the expression of ORC1 in tumor tissue and adjacent tissue was verified by immunohistochemistry (IHC).ResultsORC1 was highly expressed in the majority of tumors, and the expression level of ORC1 was associated with the pathological stages of ACC, LUAD, OV and SKCM. ORC1 was closely related with poor prognosis in ACC, LIHC, PAAD, READ and THCA. ORC1 in ACC and KICH was positively correlated with the infiltration level of immune cells while it was negatively correlated with the infiltration level of immune cells in THYM. Co-expression network analysis showed that CDCA3, GSG2, KIF2C, NCAPH and PLK1 were positively correlated with ORC1 in cancer, and enrichment analysis showed a correlation with cytosol, ATP binding and cell division. The expression of ORC1 in UCEC and KICH was higher than that in the adjacent tissues.ConclusionORC1 over-expressed in most tumors and could be severed as a novel biomarker for diagnosis. This study revealed that ORC1 might inhibit tumor immunity and might be a potential therapeutic target in cancers.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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