N-3 fatty acid intake altered fat content and fatty acid distribution in chicken breast muscle, but did not influence mRNA expression of lipid-related enzymes

Lipids in Health and Disease

Research

Nicole F Nyquist¹  Anna Haug¹  Magny Thomassen¹  Arne T Høstmark²  Tone-Kari Knutsdatter Østbye³ 

[1] Department of Animal and Aquacultural Sciences, The Norwegian University of Life Sciences, P.O. BOX 5003, Ås 1432, Norway;Institute of Health and Society, University of Oslo, P.O. BOX 113, Blindern, 0318, Oslo, Norway;Nofima, P.O. BOX 210, Osloveien 1, Ås 1430, Norway;

关键词: HMGCoA Reductase;    Fatty Acid Profile;    Desaturase Activity;    Fatty Acid Desaturases;    Breast Muscle;   

DOI  :  10.1186/1476-511X-13-92

 received in 2014-02-26, accepted in 2014-04-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe conversions of the n-3 and n-6 fatty acid of plant origin to the C20 and C22 very long chain fatty acids (LCPUFAs) is regulated by several cellular enzymes such as elongases and desaturases.MethodsSixty-five male one-day old chickens (Ross 308) were randomly divided into four groups and given one of four diets; with or without linseed oil (LO), (the diets contained equal amounts of fat) and with low or high selenium (Se). Final body weight, amount of Se and fat in breast muscle, fatty acid profile, and gene expression for fatty acid desaturases (Fads1, Fads2, Fads9), HMG-CoA reductase, Acyl-CoA oxidase (Acox), carnitine palmitoyl transferase1 (Cpt1), superoxide dismutase (Sod) and glutathione peroxidase4 (Gpx4) were analyzed in all animals, and Gpx activity in whole blood was determined.ResultsmRNA expression of elongases and desaturases in chicken breast muscle was not affected by feed rich in C18:3n-3. The highly positive correlation between amount of fat in breast muscle and the product/precursor indices of monounsaturated fatty acid synthesis, and the negative correlation between muscle fat and indices of LCPUFA synthesis should be further studied.ConclusionmRNA expression in chicken breast muscle of elongases and desaturases was not affected by feed rich in C18:3n-3. The highly positive correlation between amount of fat in breast muscle and the product/precursor indices of monounsaturated fatty acid synthesis, and the negative correlation between muscle fat and indices of LCPUFA synthesis should be further studied.

【 授权许可】

CC BY   
© Haug et al.; licensee BioMed Central Ltd. 2014