Analysis of wheat microspore embryogenesis induction by transcriptome and small RNA sequencing using the highly responsive cultivar “Svilena”

BMC Plant Biology

Research Article

Felix Seifert¹  Stefan Scholten²  Jochen Kumlehn³  Sandra Bössow⁴  Heike Gnad⁴ 

[1] Developmental Biology, Biocenter Klein Flottbek, University of Hamburg, Ohnhorststrasse 18, 22609, Hamburg, Germany;Developmental Biology, Biocenter Klein Flottbek, University of Hamburg, Ohnhorststrasse 18, 22609, Hamburg, Germany;Institute for Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70599, Stuttgart, Germany;Plant Reproductive Biology, Leibnitz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Seeland, OT Gatersleben, Germany;Saaten-Union Biotec GmbH, Am Schwabenplan 6, 06466, Seeland, OT Gatersleben, Germany;

关键词: Microspore embryogenesis induction;    Transcriptome;    Small RNA;    RNA-seq;    sRNA-seq;    Epigenetics;    Wheat;   

DOI  :  10.1186/s12870-016-0782-8

 received in 2015-12-17, accepted in 2016-04-14,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMicrospore embryogenesis describes a stress-induced reprogramming of immature male plant gametophytes to develop into embryo-like structures, which can be regenerated into doubled haploid plants after whole genome reduplication. This mechanism is of high interest for both research as well as plant breeding. The objective of this study was to characterize transcriptional changes and regulatory relationships in early stages of cold stress-induced wheat microspore embryogenesis by transcriptome and small RNA sequencing using a highly responsive cultivar.ResultsTranscriptome and small RNA sequencing was performed in a staged time-course to analyze wheat microspore embryogenesis induction. The analyzed stages were freshly harvested, untreated uninucleate microspores and the two following stages from in vitro anther culture: directly after induction by cold-stress treatment and microspores undergoing the first nuclear divisions. A de novo transcriptome assembly resulted in 29,388 contigs distributing to 20,224 putative transcripts of which 9,305 are not covered by public wheat cDNAs. Differentially expressed transcripts and small RNAs were identified for the stage transitions highlighting various processes as well as specific genes to be involved in microspore embryogenesis induction.ConclusionThis study establishes a comprehensive functional genomics resource for wheat microspore embryogenesis induction and initial understanding of molecular mechanisms involved. A large set of putative transcripts presumably specific for microspore embryogenesis induction as well as contributing processes and specific genes were identified. The results allow for a first insight in regulatory roles of small RNAs in the reprogramming of microspores towards an embryogenic cell fate.

【 授权许可】

CC BY   
© Seifert et al. 2016